Simultaneous Determination of Prasugrel and Aspirin by Second Order and Ratio First Order Derivative Ultraviolet Spectrophotometry

Table 1

Validation parameters for second order and ratio first order derivative spectrophotometric methods.

Validation parameters	Method D2*		Method RD1**
	PRA	ASP	PRA	ASP
Linearity and range (𝜇g/mL)	5–45	25–150	6–22	45–165
Wavelength (nm)	267.62	252.40	268	290
Correlation coefficient (𝑟^2)	0.99909	0.99914	0.99975	0.99972
Slope	0.000028	0.000059	0.00228	0.00714
Intercept	0.000181	−0.000276	0.00476	−0.10455
Precision (%RSD)
Repeatability	1.13%	0.91%	0.88%	0.72%
Intraday precision	0.94%–1.88%	1.09%–1.31%	0.56%–1.78%	1.14%–1.52%
Interday precision	0.92%–1.90%	0.54%–1.96%	1.05%–1.69%	1.27%–1.50%
Accuracy (%recovery)	98.81%–100.19%	99.53%–100.04%	100.07%–100.75%	99.48%–101.6%
LOD (𝜇g/mL)	0.73	2.35	0.44	1.31
LOQ (𝜇g/mL)	2.22	7.12	1.35	3.95

∗Second order derivative method. **Ratio first order derivative method.
PRA: Prasugrel; ASP: Aspirin. %RSD: percent relative standard deviation.