Research Article
Simultaneous Determination of Prasugrel and Aspirin by Second Order and Ratio First Order Derivative
Ultraviolet Spectrophotometry
Table 1
Validation parameters for second order and ratio first order derivative spectrophotometric methods.
| Validation parameters | Method D2* | Method RD1** | PRA | ASP | PRA | ASP |
| Linearity and range (g/mL) | 5–45 | 25–150 | 6–22 | 45–165 | Wavelength (nm) | 267.62 | 252.40 | 268 | 290 | Correlation coefficient () | 0.99909 | 0.99914 | 0.99975 | 0.99972 | Slope | 0.000028 | 0.000059 | 0.00228 | 0.00714 | Intercept | 0.000181 | −0.000276 | 0.00476 | −0.10455 | Precision (%RSD) | | | | | Repeatability | 1.13% | 0.91% | 0.88% | 0.72% | Intraday precision | 0.94%–1.88% | 1.09%–1.31% | 0.56%–1.78% | 1.14%–1.52% | Interday precision | 0.92%–1.90% | 0.54%–1.96% | 1.05%–1.69% | 1.27%–1.50% | Accuracy (%recovery) | 98.81%–100.19% | 99.53%–100.04% | 100.07%–100.75% | 99.48%–101.6% | LOD (g/mL) | 0.73 | 2.35 | 0.44 | 1.31 | LOQ (g/mL) | 2.22 | 7.12 | 1.35 | 3.95 |
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Second order derivative method. **Ratio first order derivative method. PRA: Prasugrel; ASP: Aspirin. %RSD: percent relative standard deviation.
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