Utilization of a Green Brominating Agent for the Spectrophotometric Determination of Pipazethate HCl in Pure Form and Pharmaceutical Preparations
Table 2
Optical characteristics and statistical data for the regression equation of the proposed methods.
Parameters
Methods
A
B
C
D
E
Wavelengths (nm)
520
610
550
510
480
Beer’s law limit (g mL−1)
0.5–3.0
0.5–5.0
1.0–8.0
0.5–4.0
0.5–3.5
Molar absorptivity , (L mol−1 cm−1) × 105
1.1114
0.6242
0.3658
1.0152
1.2558
Sandell's sensitivity (ng cm−2)
39.23
11.92
6.98
42.95
34.72
Linear regression equationa
Intercept ()
0.0063
0.0071
0.0031
0.0068
1.186
Slope ()
0.2474
0.0805
0.1402
0.2267
Correlation coefficient ()
0.9997
0.9995
0.9999
0.9998
0.9998
Recoveryb
Relative standard deviation, RSD%
0.887
1.03
0.97
1.35
0.92
Relative error, RE%
0.931
1.08
1.02
1.42
0.96
Limit of detection LOD, (g mL−1)
0.094
0.086
0.25
0.072
0.102
Limit of quantification LOQ, (g mL−1)
0.31
0.287
0.83
0.24
0.34
Calculated t-valuec
0.64
0.47
0.68
0.105
0.93
Calculated F-valuec
2.13
2.85
2.53
4.97
2.27
, where is the concentration of PZT in g mL−1, is the absorbance units, is the intercept, and is the slope.
bAverage of six determinations.
cThe theoretical values of and are 2.57 and 5.05, respectively, at confidence limit at 95% confidence level and five degrees of freedom ().