Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical Preparations
Table 1
Results of analytical parameters for proposed methods.
Method A
Method B
Method C
Linearity rangea [μg mL−1]
25–300
25–350
50–500
Molar absorptivity [l mol−1 cm−1]
3.46 × 103
2.31 × 103
1.6 × 103
Sandel’s sensitivity [μg cm−2 per 0.001 absorbance unit]
5.56 × 10−2
7.41 × 10−2
1.02 × 10−1
Precision
Intradayb, RSD %
0.75
0.82
0.56
Interdayc, RSD %
1.02
1.15
1.27
Regression equationd
Slope ± SD
0.0028 ± 0.00006
0.0024 ± 0.00002
0.0015 ± 0.00003
Intercept ± SD
0.0777 ± 0.00011
0.0263 ± 0.00024
0.0381 ± 0.00005
Correlation coefficient,
0.9996
0.9996
0.9983
LOD [μg mL−1]
0.122
0.279
0.583
LOQ [μg mL−1]
0.407
0.932
1.94
Average of six determinations.
b corresponding to replicate analysis for each level.
cResults of five different days.
d [ is the concentration of drug in μg mL−1 and is the absorbance at ].