Interaction between Chromosomal Protein HMGB1 and DNA Studied by DNA-Melting Analysis

Figure 4

The melting curves of pure HMGB1 (squares) and HMGB1 in the complex with DNA at (circles) obtained by measuring circular dichroism at 222 nm. The concentration of DNA in the sample was 30 μg/mL, and the concentration of HMGB1 was 15 μg/mL. The complex was prepared in 0.25 mM EDTA/0.2 mM NaCl solution. Optical path length was 1 cm.