Micellar Enhanced Three-Dimensional Excitation-Emission Matrix Fluorescence for Rapid Determination of Antihypertensives in Human Plasma with Aid of Second-Order Calibration Methods

Table 1

The concentration of valsartan and amlodipine besylate in calibration, test, and prediction samples.

Analytes	Calibration samples		Test samples		Prediction samples
	Number	(ng/mL)	Number	(ng/mL)	Number	(ng/mL)
Valsartan	1	78.2	12	180.4	18	90.2
	2	126.3	13	240.5	19	138.3
	3	174.3	14	300.6	20	186.4
	4	222.4	15	360.7	21	234.5
	5	270.5	16	420.8	22	282.6
	6	318.6	17	481.0	23	330.7
	7	366.7			24	378.8
	8	414.8			25	426.9
	9	462.9			26	474.9
	10	511.0			27	523.0
	11	559.1
Amlodipine besylate	28	1.93	39	3.02	45	3.38
	29	2.95	40	4.22	46	4.46
	30	3.98	41	5.43	47	5.55
	31	5.00	42	6.63	48	6.63
	32	6.03	43	7.84	49	7.72
	33	7.06	44	9.05	50	8.80
	34	8.08			51	9.89
	35	9.11			52	10.97
	36	10.13
	37	11.16
	38	12.06