Analysis of 31 Hydrazones of Carbonyl Compounds by RRLC-UV and RRLC-MS(/MS): A Comparison of Methods

Table 3

Major fragments () of MS² spectrum formed by fragmentation of the precursor [M-H]⁻ ions of the hydrazones.

Hydrazone	[M-H]^−	Fragment ions of [M-H]^− ()
		152	163	179	182	[M-H-17]^−	[M-H-30]^−	[M-H-31]^−	[M-H-47]^−	[M-H-93]^−	[M-H-164]^−
Formaldehyde	209	xx	xxx	xx
Acetaldehyde	223	xx	xxx	xxx			X
2,3-Butanedione	265	xx		xx			X		xxx
Acetone	237	xx		xx			Xxx		x
Acrolein	235	x	xxx	xx			Xx		xx
Propionaldehyde	237	xx	xxx	xx		x	Xx		x
Crotonaldehyde	249	xxx	xx	xx		x		xx	xx
Butanone	251	xx		xx			Xxx		x
Methacrolein	249	xx	xxx	xx		x		x	xx
Butyraldehyde	251	xx	xxx	xx			xx		x
Salicylaldehyde	301	x	xx		xxx				xx	x	Xx
Benzaldehyde	285	x	xxx	x			x		xx	x	Xx
Cyclohexanone	277	x		x			xxx		x
2- and 3-pentanone	265	xx		xx			xxx		x
Isovaleraldehyde	265	xx	xxx	xx			xx		x		x
Valeraldehyde	265	xx	xxx	xx			xx		x		x
p-Methoxyacetophenone	329	x		x					xxx		x
o-, m-, and p-tolualdehydes	299	x	xxx	xx					xxx	x	xx
Methyl isobutyl ketone	279	xx		x		x	xxx		x
Hexaldehyde	279	xx	xxx	xx			x		x		x
Dimethylbenzaldehyde	313		xx	x					xxx	x	xx
Heptanaldehyde	293	xx	xxx	xx		x	xx		xx		x
Octanaldehyde	307	xx	xxx	xx			x		x		x
Nonanaldehyde	321	xx	xxx	xx			xx		xx		x
Decanaldehyde	335	xx	xxx	xx		x	xx		xx		xx
Undecanaldehyde	349	xx	xxx	xx			xx		xx	x	xx
Dodecanaldehyde	363	xx	xxx	xx			xx		xx		xx
Tridecanaldehyde	377	xx	xxx	x			xx		xxx	x	xxx

Relative abundance: xxx: 100%; xx: 50%; x: ≤20%.
xxx: fragment ion selected for SRM.