Stretched Gelatin Phantom for Detection of Residual Dipolar Couplings in MR Spectra and Data Analysis of Carnosine

Table 2

The measured RDC values for carnosine from the high resolution samples and from the phantom. The first 8 rows refer to histidine residue and the bottom 4 rows refer to β-alanine residue.

Coupling pair	RDC in 20% gelatin [Hz]; 120% stretching ratio	RDC in 30% gelatin [Hz]; 140% stretching ratio	RDC in 40% gelatin [Hz]; 130% stretching ratio	RDC in phantom 30% gelatin [Hz]; 130% stretching ratio
C2-H2	−1.55	−0.5	1	—
C5-H5	0.45	3	1.5	3.5
Cα-Hα	12.55	23.5	17	16.5
Cβ-H	1.3	9	4.5	—
Cβ-H	−8.9	−13.5	−8.5	—
H-H	−6	−8	−5	—
H-Hα	−10	−14	−13	—
H-Hα	−8	−11	−9	—
Cα′-Hα′	12.5	17.5	15	—
Cβ′-Hβ′	6.6	11	7.5	—
H-H	13	25	17	—
H-H	5 or −6	12 or −14	7 or −9	—

It was not possible to achieve unambiguous determination; both values provide identical spectral pattern.