Jak2-Independent Activation of Stat3 by Intracellular Angiotensin II in Human Mesangial Cells
Figure 8
Effect of Jak2 inhibition on intracellular Ang II-induced tyrosine 705 (Tyr705) phosphorylation of Stat3. Incubation of mesangial cells with 1 μM exogenous Ang II (ex-Ang II) for 20 minutes increased (Tyr705) Stat3 phosphorylation compared to control cells incubated in 5 mM glucose (NG), and this increase was inhibited by AG-490. In contrast, transfection of mesangial cells with Ang II for 20 minutes (NG + t-Ang II) increased Stat3 (Tyr705) phosphorylation which was not blocked by AG-490. Treatment with Jak inhibitor I had no effect on Stat3 (Tyr705) phosphorylation in cells treated with exogenous Ang II or transfected with Ang II. Data are presented as mean ± SEM of observations shown in parentheses for each group. versus NG; versus NG + ex-Ang II.
