About this Journal Submit a Manuscript Table of Contents
Journal of Signal Transduction
Volume 2011 (2011), Article ID 894510, 8 pages
http://dx.doi.org/10.1155/2011/894510
Review Article

Drosophila SOCS Proteins

MRC Centre for Developmental and Biomedical Genetics and Department of Biomedical Science, The University of Sheffield, Firth Court, Sheffield S10 2TN, UK

Received 15 July 2011; Accepted 15 September 2011

Academic Editor: Karl Matter

Copyright © 2011 Wojciech J. Stec and Martin P. Zeidler. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The importance of signal transduction cascades such as the EGFR and JAK/STAT pathways for development and homeostasis is highlighted by the high levels of molecular conservation maintained between organisms as evolutionary diverged as fruit flies and humans. This conservation is also mirrored in many of the regulatory mechanisms that control the extent and duration of signalling in vivo. One group of proteins that represent important physiological regulators of both EGFR and JAK/STAT signalling is the members of the SOCS family. Only 3 SOCS-like proteins are encoded by the Drosophila genome, and despite this low complexity, Drosophila SOCS proteins share many similarities to their human homologues. SOCS36E is both a target gene and negative regulator of JAK/STAT signalling while SOCS44A and SOCS36E represent positive and negative regulators of EGFR signalling. Here we review our current understanding of Drosophila SOCS proteins, their roles in vivo, and future approaches to elucidating their functions.

1. Introduction

Signalling pathways are required for correct development as well as maintenance of homeostasis in all multicellular organisms, while misregulation of these pathways is frequently associated with a range of diseases, including cancer and associated neoplasias. To avoid such events, multiple forms of regulation have emerged with essentially every level of most signalling cascades being targeted for regulation. To assure tight control of signalling output, families of specialised proteins have evolved that can function via mechanisms including sequestration of the pathway ligands, formation of inactive receptor complexes, inhibition of kinases, or regulation of transcriptional activity. The Suppressor of Cytokine Signalling (SOCS) family has been found to regulate JAK/STAT as well as receptor tyrosine kinase signalling such as the EGFR pathway. The mammalian family of SOCS proteins consists of eight members, SOCS1–7 and CIS (reviewed elsewhere in this issue and in [1]), and each contains a centrally located SH2 domain and a SOCS box situated in the C-terminus. SOCS4–7 are characterized by long dissimilar N-terminal regions lacking any distinct domains (Figure 1(a)). By contrast, SOCS1 and 3 have short N-terminal domains that contain a kinase inhibitory region located immediately upstream of the SH2 domain. All SOCS family members bind to phosphorylated tyrosine residues via their SH2 domains; this association allows SOCS proteins to bind to phosphorylated JAKs and receptors and may act as a direct steric inhibitor preventing Signal Transducer and Activator of Transcription (STAT) molecules from associating with the activated receptor/JAK complex [1]. In addition, interactions via the SH2 domain also provide a substrate recognition function for the SOCS-box associated Elongin-Cullin-SOCS (ECS) E3 ubiquitin ligase complex. In this scenario, the SOCS-box domain interacts with Elongins B and C, which in turn recruit Cullin 5 and Roc/Rbx1 to generate a competent Ubiquitin E3 ligase complex. Docking of this complex allows the transfer of ubiquitin moieties onto the substrate molecule, targeting it for degradation.

fig1
Figure 1: Structural conservation of SOCS family proteins. (a) Schematic representation of SOCS proteins. Percentage of conserved amino acids within the regions specified is shown as is protein sizes. Red indicates the SH2 domain and SOCS-box (SB) domain is shown in green. (b) Conservation of the carboxy-terminal regions (including the SH2 and SOCS-box domains) of human and Drosophila SOCS-family proteins is shown as percentage shared identity. Numbers in brackets indicate length of the full-length protein. (c) Phylogram representing common ancestry of full-length SOCS proteins from multiple species as indicated, Drosophila SOCS proteins are in bold. Identities and phylogram shown are generated by the ClustalW2 sequence alignment analysis tool [2].

While the biochemical interactions of human SOCS proteins are being progressively elucidated, the role of these proteins in vivo is less easily determined. One system in which SOCS proteins can be readily examined in vivo is the genetically tractable Drosophila model system. Recent developments from Drosophila regarding JAK/STAT, EGFR signaling, and SOCS regulation are discussed below.

2. JAK/STAT Pathway in Drosophila

The Drosophila JAK/STAT signalling pathway is stimulated by three Unpaired-like ligands, Upd [3], Upd2 [4], and Upd3 [5]. Ligand binding to a single transmembrane receptor, Domeless (Dome) [6], causes the activation of the associated JAK termed Hopscotch (Hop) [7]. Phosphorylation of both Hop and Dome subsequently leads to the binding of STAT92E [8, 9]. Following pathway stimulation, the STAT92E transcription factor becomes phosphorylated and translocates to the nucleus, where it induces transcription of pathway target genes [1012] (reviewed in [13]). As such, conservation of pathway function between human and Drosophila systems is considerable despite lower redundancy compared to the mammalian system. Drosophila JAK/STAT signalling in vivo has been shown to be involved in multiple processes including embryonic patterning [8, 14], wing formation [15], migration of border cells during oogenesis [16, 17], maintenance of stem cells in stem cell niches [1821], eye development [22], and immune responses [23, 24].

Given these diverse roles, it is not surprising that multiple regulators of JAK/STAT pathway signalling have also been conserved between vertebrates and Drosophila. One example is the tyrosine phosphatase PTP61F, identified by RNAi screening as a potent negative regulator of pathway signalling both in and ex vivo [25, 26]. Drosophila homologues of the vertebrate Protein Inhibitor of Activated STAT (PIAS) [27, 28] and the Signal Transduction Adaptor Molecule (STAM) [29] have also been characterised.

3. Drosophila SOCS Molecules

In addition to the JAK/STAT pathway regulators described above, three SOCS family members are encoded by the Drosophila genome and are termed SOCS16D, SOCS36E, and SOCS44A on the basis of their chromosomal location (Figure 1(a)) [3032]. Sequence analysis reveals a conserved SOCS-typical domain structure, with SH2 and SOCS-box domains located in the carboxy-terminal (Figure 1(a)). As expected by analogy to vertebrate homologues, N-terminal regions do not show conservation. Based on the conserved carboxy-terminal region, SOCS36E is most homologous to hSOCS5, sharing 64% identity, and SOCS16D shows 48% and 45% identity to hSOCS6 and 7, respectively, while SOCS44A shares 34% and 33% identity with the same proteins, respectively (summarised in Figure 1(b)). The relationship of the three Drosophila SOCS-like proteins to mammalian SOCS proteins suggests common ancestry of SOCS16D and 44A, which is separate from SOCS36E. Strikingly, all Drosophila SOCS contain N-terminal regions at least 100 residues longer than hSOCS1-3, suggesting that the mammalian SOCS proteins with short N-termini may have arisen after divergence of mammals and insectas (Figure 1(c)).

While best studied in Drosophila, SOCS-like molecules have also been described in other invertebrate models including the moth, Manduca sexta [33], and the flour beetle, Tribolium [34].

4. Drosophila SOCS-Genes as Transcriptional Targets of JAK/STAT Pathway Signalling

The socs36E promoter region contains 19 putative STAT92E consensus binding sites and generates a corresponding mRNA expression pattern that closely mirrors Upd expression [31], a point highlighted by double fluorescent in situ hybridisation of upd and socs36E mRNA during embryogenesis (Figure 2(a)). Given this expression pattern, it appears that pathway downregulation elicited by SOCS36E acts as a classical negative feedback loop in a manner analogous to other vertebrate SOCS-family members [35]. Northern blot analysis has demonstrated strong expression of socs36E mRNA throughout embryogenesis, diminishing at later stages of development [30], a result in line with abundance of pathway ligands throughout early development. In flies lacking the Upd pathway ligands or the JAK kinase Hop, socs36E mRNA is largely absent [31, 32]. Conversely, mutant flies carrying the constitutively active kinase, HopTuml, or ectopically expressing Upd show increased levels of socs36E mRNA [31]. Cell culture studies have also demonstrated an increase in socs36E mRNA levels within 30 minutes of pathway stimulation and by 4 hours after stimulation, a 4.6-fold increase is detected compared to the initial expression suggesting that socs36E is a strong pathway target [12]. This fact has been utilised to generate a variety of in vivo and ex vivo reporters of JAK/STAT activity. These include the 10xSTAT-luciferase reporter containing a pentamerised 441 bp region from the first intron of socs36E to generate a total of 10 potential STAT92E binding sites. This highly sensitive reporter has been used for an RNAi genomic screen [25], and a variant expressing GFP within transgenic Drosophila (termed 10xSTAT-GFP) has also proven to be a powerful tool to report endogenous JAK/STAT pathway activity in vivo (Figure 2(b), [36]).

fig2
Figure 2: Expression of SOCS36E is a proxy for JAK/STAT pathway activity and can be used as a pathway reporter. (a) Double fluorescent in situ hybridization demonstrates the association between the expression domains of upd (top and red) and socs36E (middle and green) within a stage 13 embryo. (b) Late third instar wing imaginal disc expressing the 10xSTAT-GFP reporter construct (green) in regions of high JAK/STAT activity that correspond to upd mRNA expression domains [37]. DNA (blue) outlines wing disc morphology.

By contrast, socs44A mRNA has not been identified as a transcriptional target of STAT92E [32] and neither socs44A nor socs16D is upregulated in transcript profiling experiments following pathway stimulation [12].

5. Regulation of the JAK/STAT Cascade

Although each of the three Drosophila SOCS-family proteins contains the SH2 and SOCS domains characteristic of SOCS regulators, only SOCS36E and SOCS44A have been found to regulate JAK/STAT pathway signalling, while limited studies on SOCS16D have not indicated any involvement with the JAK/STAT cascade [32]. In addition to cell-based studies that have used knockdown of socs36E as a control [5, 25, 26, 38], considerable analysis of the roles of SOCS proteins in vivo has also been undertaken.

The JAK/STAT pathway has a role in the development of Drosophila wings and their venation, which provides a convenient readout of the pathway activity [15]. Ectopic expression of SOCS36E in the developing wing results in an outstretched wing phenotype, analogous to that observed in regulatory upd mutants [30, 39]. Moreover, defects in venation of the wing were observed, consistent with mutants lacking stat92E and hop. Ectopic expression of SOCS44A also produces venation defects that do not completely phenocopy those achieved by misexpression of SOCS36E, suggesting that the two proteins may have different functions [32]. Genetic interaction experiments also suggest different roles for socs36E and socs44A. Increased dosage of SOCS44A in flies carrying combinations of weak loss-of-function Hop alleles results in increased lethality while ectopic expression of Hop leads to lethality that can be rescued by SOCS36E [30]. This indicates that SOCS36E is a strong negative regulator of the pathway while SOCS44A can suppress signalling to a weaker extent.

More detailed in vivo analysis of SOCS36E function comes from studies of the testicular stem cell niche. The testis stem cell niche is probably the best described niche to date and JAK/STAT pathway signalling has been shown to play a crucial role in stem cell maintenance within it [18, 19, 40]. Analysis of interactions between different components of the niche have also revealed a role for SOCS36E in maintaining the correct ratio of different stem cell populations within the niche [41]. In socs36e mutant testis a loss of germline stem cells (GSC) is observed in favour of somatic stem cells, termed Cist Progenitor Cells (CPC). Moreover, increased levels of STAT92E expression are observed in CPCs and cells of the hub upon removal of SOCS36E. Conversely, overexpression of SOCS36E in the testis leads to loss of CPCs but not GSCs, suggesting that SOCS36E negatively regulates maintenance and self-renewal of CPCs, allowing for GSC self-renewal [41].

Oogenesis is another well-studied process in which JAK/STAT pathway plays an important role. Besides maintaining the stem cell balance in the ovary niche in a manner analogous to the testis [42], pathway signalling has been shown to regulate migration of the border cells in the developing egg [16, 17, 43, 44]. Expression of Upd in the paired polar cells located at the anterior and posterior tips of the follicle results in recruitment of the adjacent follicular cells to form a cluster of presumptive border cells. Eight to ten cells will migrate along the midline of the egg chamber to meet the oocyte and form the micropyle, a sperm entry point [4446]. Overexpression of SOCS36E in the border cells results in defects in recruitment and migration consistent with a reduction in JAK/STAT pathway activity [47]. SOCS44A has however not been found to be involved in oogenesis [32].

Flies carrying constitutively active HopTuml develop haematopoietic abnormalities leading to formation of black melanised tumours [48]. Although the exact mechanism of tumour development has not been resolved, evidence for aberrant proliferation and differentiation of haemocyte precursors in the lymph gland (the Drosophila equivalent of a haematopoietic niche) exists [49, 50]. Overexpression of SOCS36E in the haemocyte precursors in the lymph gland is sufficient to produce a decrease in the number and size of tumours, while RNAi-mediated ablation of SOCS36E had the converse effect [12].

Despite the multiple strands of evidence demonstrating the role of SOCS36E as a negative regulator of the JAK/STAT pathway, it has to be noted that the null socs36E mutant allele is in fact homozygous viable [51, 52]. Considering the multiple requirements for JAK/STAT pathway signalling throughout development, this might seem counterintuitive. However, other pathway regulators of JAK/STAT signalling, including negative feedback loops, are known. These include the PTP61F phosphatase [25, 26], protein inhibitors of activated STAT (PIAS), and transcriptional repressors such as Ken and Barbie (reviewed in [13]). In addition, knockout of the mouse homologue of SOCS36E, SOCS5, is also homozygous viable, fertile, and does not display any phenotype [53]. As such, it appears likely that multiple forms of inhibition have emerged that are both evolutionary conserved and mutually redundant.

6. Regulation of EGFR Signalling

Wing venation requires JAK/STAT and EGFR/MAPK signalling pathways, that have been frequently found to cross-talk in mammals [32, 5458]. The Drosophila EGFR pathway consists of four ligands (Gurken, Spitz, Argos, and Boss) that bind to three distinct receptors (DER, Torso, and Sevenless) and result in activation of the RAS-RAF-MAPK pathway (reviewed in [59]). The overall signalling pathway has been highly conserved across evolutionary time. In the mammalian system, SOCS4 and 5 negatively regulate EGFR signalling by targeting the receptor for degradation [60, 61]. As described above, ectopic expression of SOCS36E within the developing Drosophila wing produces venation defects in the adult wing which partially phenocopies loss of DER and suggests an inhibition of EGFR signalling [30]. The ability of SOCS36E to downregulate EGFR signalling is further supported by findings in the developing Drosophila eye. Specification of the eight photoreceptors (R1–R8) present within each ommatidial cluster requires intracellular signalling governed by EGFR signalling [62] with differentiation of the R7 receptor requiring an additional burst of signal in form of Sevenless (Sev) activation [62, 63]. EGFR receptor expression localizes to R1, R3, R4, R6, R7, and four ancillary cone cells, while SOCS36E is expressed in all cells with exception of R2, R5, and R7 [52]. In a socs36E mutant additional R7 receptors are recruited, while overexpression of SOCS36E is sufficient to prevent R7 cell differentiation. This demonstrates a requirement for SOCS36E in regulation of fate determination in the developing eye, a cell fate decision that does not involve JAK/STAT signalling [64]. Furthermore, misexpression of downstream components of the EGFR pathway together with SOCS36E also resulted in recruitment of additional R7 cells, indicating direct and specific interaction between SOCS36E and Sev. It has however been suggested that SOCS36E is only a weak repressor of Sev as high levels of Sevenless signalling is able to suppress the phenotypes caused by SOCS36E expression [52]. Results obtained in the wing and eye imaginal discs suggest that SOCS36E is also able to weakly inhibit EGFR pathway in these other tissues demonstrating a conserved function across species.

In addition to the role of SOCS36E, SOCS44A has also been shown to play a role in the regulation of EGFR signalling. Misexpression of SOCS44A in the developing wing produces venation defects similar to JAK/STAT loss of function as well as EGFR gain of function. Indeed, phenotypes characteristic for heterozygous mutations in ras85D and EGFR were rescued upon SOCS44A overexpression and enhanced by loss of argos, a negative regulator of the EGFR pathway. On this basis, as well as interactions between misexpressed argos and a genetic deficiency removing socs44A, it has been concluded that SOCS44A upregulates EGFR signalling in the wing [32]. However, studies in the developing eye failed to identify SOCS44A as a regulator of the EGFR pathway [52]. Considering that the presence of different EGF-like receptors is present in both tissues, these results suggest that SOCS44A may show specificity to a particular receptor. However, studies in mammalian systems suggest a different function for the SOCS44A homologue, SOCS6, which downregulates the EGFR receptor c-KIT by targeting it for degradation [65]. Ultimately, the precise interactions of Drosophila SOCS proteins in regulating both EGFR and JAK/STAT pathway signalling will require further analysis at both the genetic and biochemical levels.

7. Structural Analysis of SOCS36E

Multiple biochemical and structure-function analyses of mammalian SOCS proteins have revealed a range of different mechanisms by which they exert their pathway regulatory functions. To date, no such studies have been performed on Drosophila SOCS proteins; however, genetic analysis has highlighted the importance of the SH2 domain for correct function of SOCS36E. Ectopic expression of a protein carrying a point mutation within the SH2 domain previously shown to abolish interactions with phosphorylated tyrosine did not produce any phenotypes [30, 47, 52]. These results were not surprising considering the homology of SOCS36E to SOCS5 which has also been shown to require both the SH2 and SOCS-box domains for its function [61]. However, ectopic expression of a SOCS-box truncation of SOCS36E is sufficient to generate a wing vein phenotype that resembles the milder phenotypes generated by the wild type protein [30]. Misexpression of SOCS-box truncation is also sufficient to cause mild border cell migration defects and a decrease in ommatidial R7 cell frequency [47, 52]. Despite the lack of identifiable domains in the N-terminal region of both proteins, it seems likely that SOCS36E is able to regulate JAK/STAT signalling in a SOCS-box independent manner, possibly via competitive binding to the phosphorylated tyrosine. The structure-function relationship of SOCS44A remains to be addressed.

8. Conclusions

Signalling pathways require tight regulation to prevent outcomes harmful for development and maintenance of the organism. Acting in a context-specific manner negative regulators, like SOCS family of proteins, often act to fine-tune the signal adding to the robustness of the signal transduction pathways. Moreover, from systems biology perspective negative regulators can be viewed as integral components of the developmental machinery, allowing for precise regulation of cell fate specification, survival and death, among many other outcomes. Furthermore, multiple levels of negative regulation also introduce redundancies into the system, and as a result only mild phenotypes are observed following the loss of any one regulatory component.

Of the three SOCS proteins encoded by the Drosophila genome, SOCS36E and SOCS44A have been found to interact in different directions with both the JAK/STAT and EGFR signalling pathways (summarised in Figure 3). Homologous to mammalian SOCS5, SOCS36E has received much more attention than the two remaining fruit fly family members. Genetic as well as cellular studies have identified roles in development, spermatogenesis, oogenesis, and tumour development, establishing SOCS36E as a potent, yet redundant negative regulator of JAK/STAT pathway. Weak inhibition of EGFR signalling further indicates strong conservation of function across species. The ability of SOCS36E to negatively regulate JAK/STAT pathway activities following SOCS-box domain truncations indicates a possible additional mechanism of inhibition. It will be interesting to address the potential role of SOCS36E in the regulation of tumour formation in HopTuml flies, a system previously shown to be a good model of Drosophila leukaemia and tumourigenesis studies.

894510.fig.003
Figure 3: Schematic representation of the interaction of SOCS proteins with the JAK/STAT and EGFR pathways. Positive regulation indicated by arrows and negative regulation represented by blunt-ended arrows. Dashed line arrow indicates context-specific positive regulation.

SOCS44A has not yet been studied in detail. However our current understanding indicates its ability to weakly inhibit JAK/STAT pathway and positively regulate EGFR pathway, in a context-specific manner. This is in contrast to the function of SOCS6, the closest mammalian homologue of SOCS44A. Further studies on SOCS44A as well as SOCS16D will undoubtedly identify novel roles for the wider Drosophila SOCS family. Ultimately, the mutual in vivo interprotein relationships of the fly SOCS proteins might facilitate our understanding of the higher complexity mammalian SOCS protein interactions.

Acknowledgments

The authors wish to thank Susan Smith for helpful comments on the paper as well as Natalia Arbouzova and Richard Wells for the images shown in Figure 2. W. J. Stec holds a Cancer Research UK PhD Fellowship and M. P. Zeidler is a Cancer Research UK Senior Cancer Research Fellow.

References

  1. B. A. Croker, H. Kiu, and S. E. Nicholson, “SOCS regulation of the JAK/STAT signalling pathway,” Seminars in Cell and Developmental Biology, vol. 19, no. 4, pp. 414–422, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  2. R. Chenna, H. Sugawara, T. Koike et al., “Multiple sequence alignment with the Clustal series of programs,” Nucleic Acids Research, vol. 31, no. 13, pp. 3497–3500, 2003. View at Publisher · View at Google Scholar
  3. D. A. Harrison, P. E. McCoon, R. Binari, M. Gilman, and N. Perrimon, “Drosophila unpaired encodes a secreted protein that activates the JAK signaling pathway,” Genes and Development, vol. 12, no. 20, pp. 3252–3263, 1998.
  4. J. C. G. Hombría, S. Brown, S. Häder, and M. P. Zeidler, “Characterisation of Upd2, a Drosophila JAK/STAT pathway ligand,” Developmental Biology, vol. 288, no. 2, pp. 420–433, 2005. View at Publisher · View at Google Scholar · View at PubMed
  5. V. M. Wright, K. L. Vogt, E. Smythe, and M. P. Zeidler, “Differential activities of the Drosophila JAK/STAT pathway ligands Upd, Upd2 and Upd3,” Cellular Signalling, vol. 23, no. 5, pp. 920–927, 2011. View at Publisher · View at Google Scholar · View at PubMed
  6. S. Brown, N. Hu, and J. C. G. Hombría, “Identification of the first invertebrate interleukin JAK/STAT receptor, the Drosophila gene domeless,” Current Biology, vol. 11, no. 21, pp. 1700–1705, 2001. View at Publisher · View at Google Scholar
  7. R. Binari and N. Perrimon, “Stripe-specific regulation of pair-rule genes by hopscotch, a putative Jak family tyrosine kinase in Drosophila,” Genes and Development, vol. 8, no. 3, pp. 300–312, 1994.
  8. X. S. Hou, M. B. Melnick, and N. Perrimon, “Marelle acts downstream of the Drosophila HOP/JAK kinase and encodes a protein similar to the mammalian STATs,” Cell, vol. 84, no. 3, pp. 411–419, 1996. View at Publisher · View at Google Scholar
  9. R. Yan, S. Small, C. Desplan, C. R. Dearolf, and J. E. Darnell, “Identification of a Stat gene that functions in Drosophila development,” Cell, vol. 84, no. 3, pp. 421–430, 1996. View at Publisher · View at Google Scholar
  10. P. Karsten, I. Plischke, N. Perrimon, and M. P. Zeidler, “Mutational analysis reveals separable DNA binding and trans-activation of Drosophila STAT92E,” Cellular Signalling, vol. 18, no. 6, pp. 819–829, 2006. View at Publisher · View at Google Scholar · View at PubMed
  11. M. S. Flaherty, J. Zavadil, L. A. Ekas, and E. A. Bach, “Genome-wide expression profiling in the Drosophila eye reveals unexpected repression of Notch signaling by the JAK/STAT pathway,” Developmental Dynamics, vol. 238, no. 9, pp. 2235–2253, 2009. View at Publisher · View at Google Scholar · View at PubMed
  12. S. Bina, V. M. Wright, K. H. Fisher, M. Milo, and M. P. Zeidler, “Transcriptional targets of Drosophila JAK/STAT pathway signalling as effectors of haematopoietic tumour formation,” EMBO Reports, vol. 11, no. 3, pp. 201–207, 2010. View at Publisher · View at Google Scholar · View at PubMed
  13. N. I. Arbouzova and M. P. Zeidler, “JAK/STAT signalling in Drosophila: insights into conserved regulatory and cellular functions,” Development, vol. 133, no. 14, pp. 2605–2616, 2006. View at Publisher · View at Google Scholar · View at PubMed
  14. S. Small, A. Blair, and M. Levine, “Regulation of two pair-rule stripes by a single enhancer in the Drosophila embryo,” Developmental Biology, vol. 175, no. 2, pp. 314–324, 1996. View at Publisher · View at Google Scholar · View at PubMed
  15. R. Yan, H. Luo, J. E. Darnell, and C. R. Dearolf, “A JAK-STAT pathway regulates wing vein formation in Drosophila,” Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 12, pp. 5842–5847, 1996. View at Publisher · View at Google Scholar
  16. C. Ghiglione, O. Devergne, E. Georgenthum et al., “The Drosophila cytokine receptor Domeless controls border cell migration and epithelial polarization during oogenesis,” Development, vol. 129, no. 23, pp. 5437–5447, 2002. View at Publisher · View at Google Scholar
  17. D. L. Silver and D. J. Montell, “Paracrine signaling through the JAK/STAT pathway activates invasive behavior of ovarian epithelial cells in Drosophila,” Cell, vol. 107, no. 7, pp. 831–841, 2001. View at Publisher · View at Google Scholar
  18. A. A. Kiger, D. L. Jones, C. Schulz, M. B. Rogers, and M. T. Fuller, “Stem cell self-renewal specified by JAK-STAT activation in response to a support cell cue,” Science, vol. 294, no. 5551, pp. 2542–2545, 2001. View at Publisher · View at Google Scholar · View at PubMed
  19. N. Tulina and E. Matunis, “Control of stem cell self-renewal in Drosophila spermatogenesis by JAK-STAT signaling,” Science, vol. 294, no. 5551, pp. 2546–2549, 2001. View at Publisher · View at Google Scholar · View at PubMed
  20. W. Liu, S. R. Singh, and S. X. Hou, “JAK-STAT is restrained by Notch to control cell proliferation of the drosophila intestinal stem cells,” Journal of Cellular Biochemistry, vol. 109, no. 5, pp. 992–999, 2010. View at Publisher · View at Google Scholar · View at PubMed
  21. W. Wang, Y. Li, L. Zhou, H. Yue, and H. Luo, “Role of JAK/STAT signaling in neuroepithelial stem cell maintenance and proliferation in the Drosophila optic lobe,” Biochemical and Biophysical Research Communications, vol. 410, no. 4, pp. 714–720, 2011. View at Publisher · View at Google Scholar · View at PubMed
  22. E. A. Bach, S. Vincent, M. P. Zeidler, and N. Perrimon, “A sensitized genetic screen to identify novel regulators and components of the Drosophila janus kinase/signal transducer and activator of transcription pathway,” Genetics, vol. 165, no. 3, pp. 1149–1166, 2003.
  23. H. Agaisse and N. Perrimon, “The roles of JAK/STAT signaling in Drosophila immune responses,” Immunological Reviews, vol. 198, pp. 72–82, 2004. View at Publisher · View at Google Scholar
  24. E. J. Kwon, H. S. Park, Y. S. Kim et al., “Transcriptional regulation of the Drosophila raf proto-oncogene by drosophila STAT during development and in immune response,” Journal of Biological Chemistry, vol. 275, no. 26, pp. 19824–19830, 2000. View at Publisher · View at Google Scholar · View at PubMed
  25. G. H. Baeg, R. Zhou, and N. Perrimon, “Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila,” Genes and Development, vol. 19, no. 16, pp. 1861–1870, 2005. View at Publisher · View at Google Scholar · View at PubMed
  26. P. Müller, D. Kuttenkeuler, V. Gesellchen, M. P. Zeidler, and M. Boutros, “Identification of JAK/STAT signalling components by genome-wide RNA interference,” Nature, vol. 436, no. 7052, pp. 871–875, 2005. View at Publisher · View at Google Scholar · View at PubMed
  27. A. Betz, N. Lampen, S. Martinek, M. W. Young, and J. E. Darnell, “A Drosophila PIAS homologue negatively regulates stat92E,” Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 17, pp. 9563–9568, 2001. View at Publisher · View at Google Scholar · View at PubMed
  28. K. L. Hari, K. R. Cook, and G. H. Karpen, “The Drosophila Su(var)2-10 locus regulates chromosome structure and function and encodes a member of the PIAS protein family,” Genes and Development, vol. 15, no. 11, pp. 1334–1348, 2001. View at Publisher · View at Google Scholar · View at PubMed
  29. S. Mesilaty-Gross, A. Reich, B. Motro, and R. Wides, “The Drosophila STAM gene homolog is in a tight gene cluster, and its expression correlates to that of the adjacent gene ial,” Gene, vol. 231, no. 1-2, pp. 173–186, 1999. View at Publisher · View at Google Scholar
  30. B. A. Callus and B. Mathey-Prevot, “SOCS36E, a novel Drosophila SOCS protein, suppresses JAK/STAT and EGF-R signalling in the imaginal wing disc,” Oncogene, vol. 21, no. 31, pp. 4812–4821, 2002. View at Publisher · View at Google Scholar · View at PubMed
  31. P. Karsten, S. Häder, and M. P. Zeidler, “Cloning and expression of Drosophila SOCS36E and its potential regulation by the JAK/STAT pathway,” Mechanisms of Development, vol. 117, no. 1-2, pp. 343–346, 2002. View at Publisher · View at Google Scholar
  32. J. S. Rawlings, G. Rennebeck, S. M. W. Harrison, R. Xi, and D. A. Harrison, “Two Drosophila suppressors of cytokine signaling (SOCS) differentially regulate JAK and EGFR pathway activities,” BMC Cell Biology, vol. 5, no. 1, p. 38, 2004. View at Publisher · View at Google Scholar · View at PubMed
  33. G. C. Elliott and M. P. Zeidler, “MsSOCS expression indicates a potential role for JAK/STAT signalling in the early stages of Manduca sexta spermatogenesis,” Insect Molecular Biology, vol. 17, no. 5, pp. 475–483, 2008. View at Publisher · View at Google Scholar · View at PubMed
  34. D. Bäumer, J. Trauner, D. Hollfelder, A. Cerny, and M. Schoppmeier, “JAK-STAT signalling is required throughout telotrophic oogenesis and short-germ embryogenesis of the beetle Tribolium,” Developmental Biology, vol. 350, no. 1, pp. 169–182, 2011. View at Publisher · View at Google Scholar · View at PubMed
  35. R. Starr, T. A. Willson, E. M. Viney et al., “A family of cytokine-inducible inhibitors of signalling,” Nature, vol. 387, no. 6636, pp. 917–921, 1997. View at Publisher · View at Google Scholar · View at PubMed
  36. E. A. Bach, L. A. Ekas, A. Ayala-Camargo et al., “GFP reporters detect the activation of the Drosophila JAK/STAT pathway in vivo,” Gene Expression Patterns, vol. 7, no. 3, pp. 323–331, 2007. View at Publisher · View at Google Scholar · View at PubMed
  37. T. Mukherjee, J. C. Hombría, and M. P. Zeidler, “Opposing roles for Drosophila JAK/STAT signalling during cellular proliferation,” Oncogene, vol. 24, no. 15, pp. 2503–2511, 2005. View at Publisher · View at Google Scholar · View at PubMed
  38. O. M. Vidal, W. Stec, N. Bausek, E. Smythe, and M. P. Zeidler, “Negative regulation of Drosophila JAK-STAT signalling by endocytic trafficking,” Journal of Cell Science, vol. 123, part 20, pp. 3457–3466, 2010. View at Publisher · View at Google Scholar · View at PubMed
  39. H. J. Müller, “Types of visible variations induced by X-rays in Drosophila,” Journal of Genetics, vol. 22, no. 3, pp. 299–334, 1930. View at Publisher · View at Google Scholar
  40. J. L. Leatherman and S. Dinardo, “Zfh-1 controls somatic stem cell self-renewal in the Drosophila testis and nonautonomously influences germline stem cell self-renewal,” Cell Stem Sell, vol. 3, no. 1, pp. 44–54, 2008. View at Publisher · View at Google Scholar · View at PubMed
  41. S. R. Singh, Z. Zheng, H. Wang, S. W. Oh, X. Chen, and S. X. Hou, “Competitiveness for the niche and mutual dependence of the germline and somatic stem cells in the Drosophila testis are regulated by the JAK/STAT signaling,” Journal of Cellular Physiology, vol. 223, no. 2, pp. 500–510, 2010. View at Publisher · View at Google Scholar · View at PubMed
  42. E. Decotto and A. C. Spradling, “The Drosophila ovarian and testis stem cell niches: similar somatic stem cells and signals,” Developmental Cell, vol. 9, no. 4, pp. 501–510, 2005. View at Publisher · View at Google Scholar · View at PubMed
  43. S. Beccari, L. Teixeira, and P. Rorth, “The JAK/STAT pathway is required for border cell migration during Drosophila oogenesis,” Mechanisms of Development, vol. 111, no. 1-2, pp. 115–123, 2002. View at Publisher · View at Google Scholar
  44. R. Xi, J. R. McGregor, and D. A. Harrison, “A gradient of JAK pathway activity patterns the anterior-posterior axis of the follicular epithelium,” Developmental Cell, vol. 4, no. 2, pp. 167–177, 2003. View at Publisher · View at Google Scholar
  45. M. Grammont and K. D. Irvine, “Organizer activity of the polar cells during Drosophila oogenesis,” Development, vol. 129, no. 22, pp. 5131–5140, 2002.
  46. D. J. Montell, “Border-cell migration: the race is on,” Nature Reviews Molecular Cell Biology, vol. 4, no. 1, pp. 13–24, 2003. View at Publisher · View at Google Scholar · View at PubMed
  47. D. L. Silver, E. R. Geisbrecht, and D. J. Montell, “Requirement for JAK/STAT signaling throughout border cell migration in Drosophila,” Development, vol. 132, no. 15, pp. 3483–3492, 2005. View at Publisher · View at Google Scholar · View at PubMed
  48. H. Luo, W. P. Hanratty, and C. R. Dearolf, “An amino acid substitution in the Drosophila hop(Tum-l) Jak kinase causes leukemia-like hematopoietic defects,” EMBO Journal, vol. 14, no. 7, pp. 1412–1420, 1995.
  49. J. Krzemień, L. Dubois, R. Makki, M. Meister, A. Vincent, and M. Crozatier, “Control of blood cell homeostasis in Drosophila larvae by the posterior signalling centre,” Nature, vol. 446, no. 7133, pp. 325–328, 2007. View at Publisher · View at Google Scholar · View at PubMed
  50. R. Makki, M. Meister, D. Pennetier et al., “A short receptor downregulates JAK/STAT signalling to control the Drosophila cellular immune response,” PLoS Biology, vol. 8, no. 8, Article ID e1000441, pp. 33–34, 2010. View at Publisher · View at Google Scholar · View at PubMed
  51. H. J. Bellen, R. W. Levis, G. Liao et al., “The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes,” Genetics, vol. 167, no. 2, pp. 761–781, 2004. View at Publisher · View at Google Scholar · View at PubMed
  52. I. Almudi, H. Stocker, E. Hafen, M. Corominas, and F. Serras, “SOCS36E specifically interferes with sevenless signaling during Drosophila eye development,” Developmental Biology, vol. 326, no. 1, pp. 212–223, 2009. View at Publisher · View at Google Scholar · View at PubMed
  53. C. Brender, R. Columbus, D. Metcalf et al., “SOCS5 is expressed in primary B and T lymphoid cells but is dispensable for lymphocyte production and function,” Molecular and Cellular Biology, vol. 24, no. 13, pp. 6094–6103, 2004. View at Publisher · View at Google Scholar · View at PubMed
  54. P. C. Heinrich, I. Behrmann, S. Haan, H. M. Hermanns, G. Müller-Newen, and F. Schaper, “Principles of interleukin (IL)-6-type cytokine signalling and its regulation,” Biochemical Journal, vol. 374, part 1, pp. 1–20, 2003. View at Publisher · View at Google Scholar · View at PubMed
  55. K. Shuai and B. Liu, “Regulation of JAK-STAT signalling in the immune system,” Nature Reviews Immunology, vol. 3, no. 11, pp. 900–911, 2003.
  56. S. G. Rane and E. P. Reddy, “Janus kinases: components of multiple signaling pathways,” Oncogene, vol. 19, no. 49, pp. 5662–5679, 2000.
  57. J. F. de Celis and F. J. Diaz-Benjumea, “Developmental basis for vein pattern variations in insect wings,” International Journal of Developmental Biology, vol. 47, no. 7-8, pp. 653–663, 2003.
  58. B. Z. Shilo, “Signaling by the Drosophila epidermal growth factor receptor pathway during development,” Experimental Cell Research, vol. 284, no. 1, pp. 140–149, 2003. View at Publisher · View at Google Scholar
  59. M. A. Simon, “Receptor tyrosine kinases: specific outcomes from general signals,” Cell, vol. 103, no. 1, pp. 13–15, 2000.
  60. A. N. Bullock, M. C. Rodriguez, J. E. Debreczeni, Z. Songyang, and S. Knapp, “Structure of the SOCS4-elonginB/C complex reveals a distinct SOCS box interface and the molecular basis for SOCS-dependent EGFR degradation,” Structure, vol. 15, no. 11, pp. 1493–1504, 2007. View at Publisher · View at Google Scholar · View at PubMed
  61. E. Kario, M. D. Marmor, K. Adamsky et al., “Suppressors of cytokine signaling 4 and 5 regulate epidermal growth factor receptor signaling,” Journal of Biological Chemistry, vol. 280, no. 8, pp. 7038–7048, 2005. View at Publisher · View at Google Scholar · View at PubMed
  62. M. Freeman, “Reiterative use of the EGF receptor triggers differentiation of all cell types in the Drosophila eye,” Cell, vol. 87, no. 4, pp. 651–660, 1996. View at Publisher · View at Google Scholar
  63. M. A. Simon, D. D. L. Bowtell, G. S. Dodson, T. R. Laverty, and G. M. Rubin, “Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase,” Cell, vol. 67, no. 4, pp. 701–716, 1991.
  64. M. P. Zeidler, N. Perrimon, and D. I. Strutt, “Polarity determination in the Drosophila eye: a novel role for unpaired and JAK/STAT signaling,” Genes and Development, vol. 13, no. 10, pp. 1342–1353, 1999.
  65. F. Zadjali, A. C. W. Pike, M. Vesterlund et al., “Structural basis for c-KIT inhibition by the suppressor of cytokine signaling 6 (SOCS6) ubiquitin ligase,” Journal of Biological Chemistry, vol. 286, no. 1, pp. 480–490, 2011. View at Publisher · View at Google Scholar · View at PubMed