Prolactin and Dexamethasone Regulate Second Messenger-Stimulated Cl⁻ Secretion in Mammary Epithelia

Figure 3

Effect of prolactin and/or dexamethasone (a) DPC and furosemide ((b) and (c)) treatment on cumulative iodide efflux. (a) HC-11 cells were pretreated with 1 μg/mL prolactin (P) and/or 1 μg/mL dexamethasone (D) for 24 h prior to iodide efflux assay ((b) and (c)) effect of 10 μM DPC (a CFTR inhibitor) and 10 μM furosemide (a NKCC1 inhibitor) on iodide efflux in control (b) and 24 h P-D-treated cells (c). Data represent mean ± SEM relative to value at starting point. 𝑛≥3. *𝑃<0.05 versus non-inhibitor treated groups.

(a)

(b)

(c)