Dopamine D₂ Receptor-Mediated Heterologous Sensitization of AC5 Requires Signalosome Assembly

Figure 1

Gα _s-CFP mutants are functional and rescue heterologous sensitization of AC5. (a) Schematic of Gα _s-CFP constructs. (b) Acute cAMP accumulation in cells expressing AC5 and D₂ alone (ctrl) or in combination with 10 ng Gα _s-CFP (wild type, C3S, or IEK+) was measured under basal (open bars) or forskolin-stimulated conditions (black bars). **=𝑃<0.01, *= 𝑃<0.05, using a paired, one-tailed t-test comparing basal and forskolin-stimulated values. (c) Expression and localization of Gα _s-CFP mutants. (d) Heterologous sensitization of AC5 in cells expressing AC5 and D₂ in the absence or presence of Gα _s-CFP. Data shown represent fold-increase of cAMP accumulation observed in quinpirole-treated cells. **=𝑃<0.01, *= 𝑃<0.05, using a one-sample, two-tailed t-test comparing ctrl to each Gα _s-CFP construct.

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