Research Article

The Concept of Divergent Targeting through the Activation and Inhibition of Receptors as a Novel Chemotherapeutic Strategy: Signaling Responses to Strong DNA-Reactive Combinatorial Mimicries

Figure 5

Dose-dependent changes in p53 phosphorylation (Ser15) and expression in U87MG (a), U87/EGFR (b), and U87/EGFRvIII (c) glioma cells treated for 48 h. Cell lysates (40 μg) were fractionated by SDS-PAGE and probed with antiphospho-p53 (1 : 1000) antibodies (see Materials and Methods for details). Blots were subsequently stripped and reprobed for total p53 (1 : 1000) followed by α-actinin (1 : 1500). Major protein bands of 53 (p53) and 100 kDa (α-actinin) were obtained.
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