Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage
Figure 3
Expression of calcium channel subunits in satellite cells by RT-qPCR. (a) The α2δ1 subunit is expressed at significantly higher levels than the α1, β, and γ subunits in freshly isolated unsorted satellite cells. Expression of α1, β, and γ subunits was normalized to α2δ1 levels for comparison. The asterisk indicates statistically significant difference with all other data points (). (b) Temporal expression of calcium channel subunits in satellite cells cultured for different times in 20% serum. For each subunit, the fold change was calculated with their respective level at D0. The α2δ1 subunit showed a smaller fold change with time in culture when compared to the other three subunits, followed by the β subunit. The α1 and the γ subunits showed a large fold change of expression. Bars represent mean ± sem of three independent cultures measured in triplicate in the qPCR plate. Differences between data points are represented by lines. The asterisk represents differences with all other bars except those ones with an asterisk. For all graphs, the value was <0.05. All genes were referenced to the geometric mean of at least two control genes selected from YWHAZ, 18S, and HPRT1.
