Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage
Figure 5
Expression of myogenic transcription factors in satellite cells. (a) Expression levels of MyoD and myogenin were measured in the four subpopulations after two days in culture. Cells expressing α2δ1+ had significantly larger expression of myogenin than MyoD, while α2δ1− cells expressed larger levels of MyoD than myogenin. Statistical differences between data points are represented by lines (). The asterisks represent significant differences of that value with respect to all other data points. (b) The ratio of myogenin to MyoD was significantly higher in α2δ1+-expressing cells, indicating a more advanced differentiation into myotubes than α2δ1− cells. values ranged from <0.01 to <0.001.