Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and NO_i Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway

Figure 4

AKT activation is mediated by FAK. (a) NRVMs were infected (5 moi, 6–48 h) with Adv-expressing neβgal or CD2-FAK. Cell extracts (50 μg total protein per lane) were then separated by SDS-PAGE and Western blotting with antibodies specific for FAK phosphorylated at Y397, total FAK, AKT phosphorylated at S473, and total AKT. (b) The quantitative results of 9 experiments are depicted. *𝑃<0.05 versus time-matched, Adv-neβgal cells.

(a)

(b)