Increased Cell-Matrix Adhesion upon Constitutive Activation of Rho Proteins by Cytotoxic Necrotizing Factors from E. coli and Y. pseudotuberculosis
Figure 4
CNF1/CNFy-induced formation of focal adhesions. Focal adhesions in HeLa cells treated with the indicated CNF toxins or buffer were stained for the FA marker vinculin (a) or pS144/141-PAK1/2 (b) using immunocytochemistry. The number of vinculin-positive (a) or pS144/141-PAK1/2-positive FAs (b) per total cells was analysed in three independent experiments (each experiment cells). values <0.05 (*) were considered as statistically significant as compared with nontreated cells.
