Mitochondrial Oxidative Stress due to Complex I Dysfunction Promotes Fibroblast Activation and Melanoma Cell Invasiveness
Figure 4
mRNA levels of VEGF-A, SDF-1, and HGF secreted by fibroblasts cultured in hypoxic condition. (a–c) Quantitative real-time reverse transcription PCR of RNA extracted from fibroblasts cultured for 24 hours in low glucose serum-free medium in hypoxic conditions using primers for human VEGF-A (a), SDF-1 (b), and HGF (c) and GAPDH gene. Results were normalized first to GADPH expression levels and then displayed relative to level in HFY cells. Data are representative of three independent experiments. * mutated fibroblasts versus control fibroblasts.