Nucleic Acids in Human Glioma Treatment: Innovative Approaches and Recent Results

Figure 3

Scheme of SELEX technology. The starting point of the SELEX technology for aptamers production is the synthesis of a high complexity ssDNA/RNA library containing a variable region flanked by two constant regions. At each SELEX round the library is incubated with the target molecule and bound aptamers are recovered and amplified. At the end of the selection process, the PCR products are cloned and sequenced.