An Fc Gamma Receptor-Mediated Upregulation of the Production of Interleukin 10 by Intravenous Immunoglobulin in Bone-Marrow-Derived Mouse Dendritic Cells Stimulated with Lipopolysaccharide In Vitro

Figure 2

IVIG increased IL-10 mRNA transcription whereas decreased IL-12 mRNA transcription in BMDC stimulated with LPS. Cells were stimulated with LPS (1 μg/mL) in the presence of IVIG for 6 h. The expressions of mRNA for IL-10, IL-12a, and IL-12b were determined by real-time quantitative RT-PCR. Results were expressed as mean ± SEM (𝑛=3). ^##𝑃<0.01, significantly different from the medium alone (without LPS stimulation, Student’s t-test); ^∗𝑃<0.05, ^∗∗𝑃<0.01, significantly different from the Control (LPS stimulation without IVIG, Dunnett’s multiple comparison test). At least three independent experiments were conducted and representative results were shown. (a) Expression of IL-10; (b) expression of IL-12a; (c) expression of IL-12b.

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