TGF-β Signaling Cooperates with AT Motif-Binding Factor-1 for Repression of the α-Fetoprotein Promoter

Table 1

PCR primers to amplify human cDNAs.

Human cDNA		Sequence	𝐿	AT	Cycle
AFP	(+)	ATCCAGGAGAGCCAAGCATT	434	58°C	20
	(−)	TTCATCGTTTGCAGCGCTAC
ATBF1	(+)	TGGCATCAAGTACAGCGCTC	392	53°C	35
	(−)	GAACAGTTGTGCTGGGCAGA
TMEPAI	(+)	GATCATCATCATCGTGGTGG	455	60°C	35
	(−)	CACTGTCGAAGATGGTTCTG
𝛽-actin	(+)	CACCCACACTGTGCCCATCTACGA	410	63°C	22
	(−)	TGGCGTACAGGTCTTTGCGGATGT

𝐿: length of PCR fragment; AT: annealing temperature.