]>Molecular and Biochemical Effects of a Kola Nut Extract on Androgen Receptor-Mediated Pathways : Figure 5
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Figure 5: Modulation of prostate cell proliferation by Bizzy nut extracts. LNCaP or DU145 cells were grown in the presence of 0–1000 ppm Biz-2 for 24 hours, and cell growth was determined using (a) 3[H] thymidine incorporation, (b) MTT cell viability assay and tryphan blue dye staining, and (c) cytotoxicity of Biz-2 in LNCaP cells that was performed as described in Section 2. Growth inhibitory activity of the extracts was determined as [ ( A 4 9 0 ( c o n t r o l ) A 4 9 0 ( e x t r a c t ) ) / A 4 9 0 ( c o n t r o l ) ] × 1 0 0 . Each value represents mean ± SEM for three experiments performed in eight wells of a 96-well plate. Values followed by 𝑃 < . 0 5 differed significantly from the controls.