Research Article

Allergic Responses Induced by a Fungal Biopesticide Metarhizium anisopliae and House Dust Mite Are Compared in a Mouse Model

Table 1

Summary of endpoints based on the lowest dose that induced a significant response compared to the 0 μg dosea and the magnitude of the response at that dose.

Source Endpoint Relative potency
Lowest dose for significant responsea (response magnitude)
MACAHDM

BALFTotal protein (μg/mL)
LDH activity (U/l) NSb
Total cell count (×104 cells/mL) >
Macrophage count (×104 cells/mL) >
Neutrophil count (×104 cells/mL)
Eosinophil count (×104 cells/mL)
Lymphocyte count (×104 cells/mL) >
Total IgE (ng/mL) NS

SerumTotal IgE (ng/mL) >
Antigen-specific Releasec (% Total mediator release) ~
Antigen-specific IgEd (μg extract) 16.16 ~ 13.17

a Dose (μg extract) resulting in a significant increase over 0 μg dose control at .
b NS: no significant dose compared to 0 μg dose control.
c For antigen-specific IgE, dose comparison was made to the 2.5 μg dose.
d Calculated dose that would induce sufficient antigen-specific IgE resulting in 10% of total mediator release in RBL assay.