Domoic Acid-Induced Neurotoxicity Is Mainly Mediated by the AMPA/KA Receptor: Comparison between Immature and Mature Primary Cultures of Neurons and Glial Cells from Rat Cerebellum

Figure 2

Changes in the mRNA levels of neural receptors in primary cultures of CGCs exposed to domoic acid (5 μM, 10 μM, and 20 μM), at 1 DIV (immature cultures) or at 7 DIV (mature cultures) for 3 or 10 days. Note the significant downregulation of (a) NMDA-R in immature cultures after 10 days of exposure (20 μM), (b) NMDA-R in mature cultures, (e) GABA_A-R in immature cultures after 3 days of exposure (20 μM), and (f) GABA_A-R in mature cultures after 10 days of exposure (20 μM). There were no changes for (c) AMPA-R in immature cultures and (d) AMPA-R in mature cultures. Gene expression levels were normalized to the standard calibrator, the housekeeping gene 18S rRNA and the mRNA expression at 1 DIV. Data represent mean ± S.E.M. of three independent experiments performed in duplicates. ⁺𝑃<0.05  ⁺⁺𝑃<0.01  ⁺⁺⁺𝑃<0.001 comparing control cultures at 3 and 10 days. *𝑃<0.05  **𝑃<0.01  ***𝑃<0.001 comparing treated to control (untreated) cultures.

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