Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease

Figure 3

Early-Stage ALD leads to a significant increase in protein ubiquitination. (a) Immunohistochemical analysis reveals marked staining of ubiquitinated proteins in zone 3 and zone 2 of ethanol-fed mice following 6-week ingestion. PT: portal triad; CV: central vein. (𝑛=4 pairs; **𝑃<0.01). Magnification, 400x; Scale bar represents approximately 50 μm. (b) Hepatic proteasome activity revealed no change at the week 6 time point (𝑛=6 pairs; 𝑃=n.s.).

(a)

(b)