Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease

Table 3

Ethanol ingestion leads to decreased GSH and altered GSH metabolism. Reduced GSH is significantly decreased following ethanol consumption; the activities of GSH cycling and metabolizing enzymes show significant fluctuations, consistent with sustained oxidative stress (𝑛=6; ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001).

Parameter	Week 1		Week 3		Week 6
	Control	Ethanol	Control	Ethanol	Control	Ethanol
GSH (μmol/g tissue)	2.667 ± 0.145	2.421 ± 0.134	2.283 ± 0.065	2.046 ± 0.185	3.367 ± 0.239	2.516 ± 0.157^*
GSSG (μmol/g tissue)	0.238 ± 0.012	0.169 ± 0.006	0.100 ± 0.008	0.118 ± 0.012	0.173 ± 0.006	0.155 ± 0.008
GR activity (U/mg protein)	0.981 ± 0.019	0.958 ± 0.027	0.890 ± 0.040	0.850 ± 0.020	0.879 ± 0.028	1.149 ± 0.047^**
GCL activity (U/mg protein)	0.899 ± 0.042	1.230 ± 0.025^***	1.008 ± 0.032	1.154 ± 0.063	1.029 ± 0.052	1.449 ± 0.083^***
CBS activity (U/mg protein)	10.335 ± 1.285	8.459 ± 0.648	9.136 ± 0.873	9.415 ± 0.554	4.931 ± 0.224	3.549 ± 0.0812^***