Caspase-10 Is the Key Initiator Caspase Involved in Tributyltin-Mediated Apoptosis in Human Immune Cells

<table>Caspase activity induced by TBT treatment in Jurkat A3 cells. Jurkat cells were pretreated with the caspase-10 inhibitor AEVD (8.7 <i>μ</i>M, 1 h) before TBT was added (1 <i>μ</i>M, 4 h). At the end of the treatment, FAM-VAD-fmk was added as a caspase substrate that exhibits fluorescence after cleavage. Cells were incubated for further 60 min, counterstained with propidium iodide (red fluorescence), and intracellular fluorescence intensity was analysed by microscopy. C: vehicle-treated control cells; TBT: 1 <i>μ</i>M TBT; TBT + AEVD: pretreated with zAEVD-fmk for 1 h and 1 <i>μ</i>M TBT for further 4 h.</table>

Journal of Toxicology

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Figure 4

Figure 4: Caspase-10 Is the Key Initiator Caspase Involved in Tributyltin-Mediated Apoptosis in Human Immune Cells 