Protective Effect of Scutellaria litwinowii Extract on Serum/Glucose-Deprived Cultured PC12 Cells and Determining the Role of Reactive Oxygen Species

Figure 2

Flow cytometry with DCFH-DA staining for measuring ROS production for cultured PC12 cells in control group, serum/glucose deprivation alone group, and pretreatment with S. litwinowii (15–250 μg/mL) plus exposure to serum/glucose deprivation. In the pretreatment groups, the PC12 cells were exposed to serum/glucose deprivation in the presence of S. litwinowii (15–250 μg/mL) for only 24 h. The ROS production was assessed according to changes in the fluorescence intensity of DCF, the oxidation product of DCFH-DA. The data presented are means ± SEM of three independent experiments (𝑛=3). *: P value <0.001 relative to control group; ^†: P value <0.05 relative to SGD.