Retargeting Clostridium difficile Toxin B to Neuronal Cells as a Potential Vehicle for Cytosolic Delivery of Therapeutic Biomolecules to Treat Botulism

Figure 2

AGT-tag expressed at the N-terminus of TcdB retains enzymatic activity. Recombinant TcdB and AGT-TcdB were expressed and purified. 250 ng aliquots of purified TcdB and AGT-TcdB (± autobiotinylation) were analyzed by Western blots using anti-AGT-tag sera (left) and by anti-TcdB sera (middle). The positions of molecular weight markers are indicated with arrows. Each of the protein preparations was subjected to enzymatic reactions with BG-biotin and analyzed for bound biotin by Western blot with streptavidin (right). The data shown are representative of 2 independent experiments.