Differential Effects of Methyl-4-Phenylpyridinium Ion, Rotenone, and Paraquat on Differentiated SH-SY5Y Cells

Figure 5

CHX, GBR 12909, and L-NAME decreased PQ-induced LDH release into the medium. Differentiated SH-SY5Y cells were pretreated with GBR 12909 (1 μM), CHX (1,8 nM), and L-NAME (1 μM) 30 minutes prior to cultures exposure to PQ for 72 h. The delta increase in LDH release in units per liter (U/L) into the medium was calculated by subtraction of the respective controls (results were pooled from 3 different experiments, each experiment having 6 different culture wells per condition). The means were compared using one-way ANOVA, followed by Student-Newman-Keuls post hoc test (***𝑃<0.001 PQ versus control; ⁺⁺𝑃<0.01, ⁺⁺⁺𝑃<0.001 PQ versus PQ plus protector).