Expression of Glutathione Peroxidase and Glutathione Reductase and Level of Free Radical Processes under Toxic Hepatitis in Rats

Table 1

Primers of genes Gpx1, Gsr, βAct, Alb, and L3 used for real-time PCR.

Primer	Sequence	Sequences source in NCBI	Efficiency amplification, %	Generally Tm, °C
Gpx1 (forward); Gpx1 (reverse)	AGTTCGGACATCAGGAGAATGGCA; TCACCATTCACCTCGCACTTCTCA	NM_030826.3	86.3	68
Gsr (forward); Gsr (reverse)	GGAAGTCAACGGGAAGAAGTTCACTG; CAATGTAACCGGCACCCACAATAAC	NM_053906.1	91.28	64
βAct (forward); βAct (reverse)	TGTTTGAGACCTTCAACACC; TAGGAGCCAGGGCAGTAATC	NM_031144.2	94.9	63
Alb (forward); Alb (reverse)	GCAAACTGCAGGCTTGCTGTGATA; AAGTCAGCAGCTATTGAGGGCAGA	NM_134326	98.03	60
L3 (forward); L3 (reverse)	GCATTCATGGGACCGCTCAA; AGCTGCACAAAGTACTCCTGGCAT	NM_198753	89.23	68