956404.fig.002a
(a)
956404.fig.002b
(b)
Figure 2: (a) Differential effect of NMTMB on survival and NBD-PE accumulation in dog and rat hepatocytes. Cells were treated with compound in NBD-PE containing media. After 24 h cells were fixed with formaldehyde/Hoechst, and both survival and NBD-PE accumulation were quantified using the Cellomics Arrayscan instrument (as described in Section 2). Expressed as % control ±SD for 3 replicates from single isolations. Dog and rat hepatocytes were isolated for this experiment on the same day. (b) Differential effect of NMTMB on accumulation of NBD-PE in rat and dog hepatocytes. Cells were treated with NMTMB in NBD-PE containing media. After 24 h cells were fixed with formaldehyde/Hoechst and imaged using the Cellomics Arrayscan instrument. Image exposures were identical in all experiments (0.5 sec); background levels of NBD-PE accumulation were similar between dog and rat hepatocytes (approximately 50 mean average intensity units). Some artifactual accumulation of NBD-PE in dog preparations is apparent in the control sample. Dog and rat hepatocytes were isolated for this experiment on the same day.