Botulinum Toxin Suppression of CNS Network Activity In Vitro

Figure 3

Temporal evolution of spike and burst rates per minute under the influence of 50 ng/mL BoNT-A. Each data point represents a one-minute average of spike rate (top trace, left ordinate) and burst rate (lower trace, right ordinate). 40 μM bicuculline was added at 83 min to increase activity and reduce minute-to-minute activity fluctuations. A stable reference state was established at 860 spikes/min and all decreases were expressed as percent of this reference state. BoNT-A was added at 124 min and resulted in an irreversible decay of activity starting after a delay of approximately 80 min. Bursting ceased at 400 min with only low levels of residual spiking remaining.