Circadian Rhythms and Breast Cancer: The Role of Per2 in Doxorubicin-Induced Cell Death

Figure 7

Determining the role of Per2 in caspase-dependent apoptosis. MDA-MB-231 cells were subjected to (1) control conditions (C), (2) 2.5 μM doxorubicin (D), (3) 30 nM Per2 esiRNA (P), and (4) 30 nM Per2 for 48 hours + 2.5 μM Dox for 24 hours (PD). Whole cell lysates were subjected to immunoblotting with appropriate antibodies for cleaved caspase-3 (a) and PARP (b). Statistical analysis: one way ANOVA with Bonferroni post hoc correction. All results are presented as mean ± SEM (). versus control and versus control.

(a)

(b)