MeHg Developing Exposure Causes DNA Double-Strand Breaks and Elicits Cell Cycle Arrest in Spinal Cord Cells

Figure 3

Effects of MeHg on the spinal cord layers of embryos at E10. Proliferating cells labeled with anti-phosphohistone H3 (arrows) were observed in the ependymal, mantle, and marginal layers of the control and MeHg-treated embryos. The square in the ependymal control embryo represents the negative control of immunohistochemical reaction. The accompanying graph displays the NA of proliferating cells, obtained by stereological analysis, in each layer of the spinal cord. Data are presented as mean ± SEM. . Scale bar: 10 μm.