MeHg Developing Exposure Causes DNA Double-Strand Breaks and Elicits Cell Cycle Arrest in Spinal Cord Cells

Figure 4

Cell cycle proteins analyzed by immunohistochemistry and flow cytometry. Immunohistochemistry revealed p21 and cyclin E-positive cells (arrows) in the control and MeHg-treated embryos. The square in the ependymal control embryos represents the negative control of immunohistochemical reaction. The graphs display the expression profile and relative frequency of positive cells of p21 and cyclin E in the spinal cord of the control and MeHg-treated embryos. For each treatment 1,800 events were analyzed per antibody. Scale bars: 10 μm.