Neuroprotective Effects of Alpha-Mangostin on MPP⁺-Induced Apoptotic Cell Death in Neuroblastoma SH-SY5Y Cells

Figure 4

Effect of alpha-mangostin on the MPP⁺-induced apoptosis in SH-SY5Y cells. Cells were exposed to 10 μM of alpha-mangostin (α-M), 1000 μM MPP⁺, or a combination of α-M and MPP⁺ for 24 hours. Apoptosis was evaluated by staining cells with annexin-V. Flow cytometry profile represents annexin-V-FITC staining in -axis and PI in -axis (a). The number in the right upper quadrant (annexin-V⁺/PI⁺ cells) represents the percentage of late apoptotic/necrotic cells in each condition. Percentage of annexin-V-positive cells was calculated (b). Data are expressed as mean ± SEM () of percentage to untreated cells. .

(a)

(b)