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Journal of Toxicology
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Journal of Toxicology
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2016
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Article
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Tab 1
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Research Article
Chronic Exposure to Arsenic in Drinking Water Causes Alterations in Locomotor Activity and Decreases Striatal mRNA for the D2 Dopamine Receptor in CD1 Male Mice
Table 1
Primer sets used for SYBR green-based qPCR analysis of dopamine receptors.
Target
Abbreviation
Primer
Primer sequence
Primer length (nt)
Amplicon size (bp)
Gene bank accession number
Dopamine receptor D1a
Drd1
Forward
5′ CAG TCC ATG CCA AGA ATT GCC AGA 3′
24
255
NM_010076.3
Reverse
5′ AAT CGA TGC AGA ATG GCT GGG TCT 3′
24
Dopamine receptor D2
Drd2
Forward
5′ TGA ACA GGC GGA GAA TGG 3′
18
70
NM_010077.2
Reverse
5′ CTG GTG CTT GAC AGC ATC TC 3′
20
Beta-actin
Bact
Forward
5′ CCA GGT CAT CAC TAT TGG CAA CGA G 3′
25
141
NM_007393.3
Reverse
5′ TCT TTA CGG ATG TCA ACG TCA CAC T 3′
25
nt: nucleotide, bp: base pair.