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Journal of Tropical Medicine
Volume 2012 (2012), Article ID 340538, 9 pages
Towards Point-of-Care Diagnostic and Staging Tools for Human African Trypanosomiaisis
1School of Veterinary Medicine, Makerere University, P.O. Box 7062, Kampala, Uganda
2Foundation for Innovative New Diagnostics, Budé 16, Avenue 1202, Geneva, Switzerland
3School of Veterinary Sciences, The University of Queensland, Gatton Campus, Brisbane, QLD 4343, Australia
Received 19 July 2011; Accepted 17 January 2012
Academic Editor: Emad William Mohareb
Copyright © 2012 Enock Matovu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- M. P. Barrett, “The rise and fall of sleeping sickness,” Lancet, vol. 367, no. 9520, pp. 1377–1378, 2006.
- World Health Organisation, “Human African Trypanosomiasis (Sleeping Sickness): epidemiological update,” Weekly Epidemiological Record, vol. 81, no. 4, pp. 71–80, 2006.
- P. P. Simarro, J. Jannin, and P. Cattand, “Eliminating human African trypanosomiasis: where do we stand and what comes next?” PLoS Medicine, vol. 5, no. 2, article e55, 2008.
- P. P. Simarro, G. Cecchi, M. Paone et al., “The Atlas of human African trypanosomiasis: a contribution to global mapping of neglected tropical diseases,” International Journal of Health Geographics, vol. 9, article no. 57, 2010.
- M. Odiit, F. Kansiime, and J. C. K. Enyaru, “Duration of symptoms and case fatality of sleeping sickness caused by Trypanosoma brucei rhodesiense in Tororo, Uganda,” East African Medical Journal, vol. 74, no. 12, pp. 792–795, 1997.
- World Health Organization, “The global burden of disease: 2004 update,” 2008, http://www.who.int/healthinfo/global_burden_disease/en/.
- P. J. Hotez, D. H. Molyneux, A. Fenwick, E. Ottesen, S. E. Sachs, and J. D. Sachs, “Incorporating a rapid-impact package for neglected tropical diseases with programs for HIV/AIDS, tuberculosis, and malaria: a comprehensive pro-poor health policy and strategy for the developing world,” PLoS Medicine, vol. 3, no. 5, article e102, 2006.
- E. M. Fèvre, M. Odiit, P. G. Coleman, M. E. J. Woolhouse, and S. C. Welburn, “Estimating the burden of rhodesiense sleeping sickness during an outbreak in Serere, eastern Uganda,” BMC Public Health, vol. 8, article no. 96, 2008.
- E. Torreele, B. Bourdin Trunz, D. Tweats et al., “Fexinidazole—a new oral nitroimidazole drug candidate entering clinical development for the treatment of sleeping sickness,” PLoS neglected tropical diseases, vol. 4, no. 12, article e923, 2010.
- R. T. Jacobs, B. Nare, S. A. Wring et al., “Scyx-7158, an orally-active benzoxaborole for the treatment of stage 2 human african trypanosomiasis,” PLoS Neglected Tropical Diseases, vol. 5, no. 6, article e1151, 2011.
- F. Chappuis, L. Loutan, P. Simarro, V. Lejon, and P. Büscher, “Options for field diagnosis of human African trypanosomiasis,” Clinical Microbiology Reviews, vol. 18, no. 1, pp. 133–146, 2005.
- E. Magnus, T. Vervoort, and N. Van Meirvenne, “A card-agglutination test with stained trypanosomes (C.A.T.T.) for the serological diagnosis of T. b. gambiense trypanosomiasis,” Annales de la Societe Belge de Medecine Tropicale, vol. 58, no. 3, pp. 169–176, 1978.
- P. Dukes, W. C. Gibson, J. K. Gashumba et al., “Absence of the LiTat 1.3 (CATT antigen) gene in Trypanosoma brucei gambiense stocks from Cameroon,” Acta Tropica, vol. 51, no. 2, pp. 123–134, 1992.
- J. C. K. Enyaru, E. Matovu, M. Akol et al., “Parasitological detection of Trypanosoma brucei gambiense in serologically negative sleeping-sickness suspects from north-western Uganda,” Annals of Tropical Medicine and Parasitology, vol. 92, no. 8, pp. 845–850, 1998.
- E. Hasker, P. Mitashi, R. Baelmans et al., “A new format of the CATT test for the detection of Human African Trypanosomiasis, designed for use in peripheral health facilities,” Tropical Medicine and International Health, vol. 15, no. 2, pp. 263–267, 2010.
- F. Checchi, F. Chappuis, U. Karunakara, G. Priotto, and D. Chandramohan, “Accuracy of five algorithms to diagnose gambiense human african trypanosomiasis,” PLoS Neglected Tropical Diseases, vol. 5, no. 7, article e1233, 2011.
- P. T. Woo, “Evaluation of the haematocrit centrifuge and other techniques for the field diagnosis of human trypanosomiasis and filariasis,” Acta Tropica, vol. 28, no. 3, pp. 298–303, 1971.
- W. H. R. Lumsden, C. D. Kimber, D. A. Evans, and S. J. Doig, “Trypanosoma brucei: miniature anion-exchange centrifugation technique for detection of low parasitaemias: adaptation for field use,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 73, no. 3, pp. 312–317, 1979.
- P. Büscher, D. M. Ngoyi, J. Kaboré et al., “Improved models of mini anion exchange centrifugation technique (mAECT) and modified single centrifugation (MSC) for sleeping sickness diagnosis and staging,” PLoS Neglected Tropical Diseases, vol. 3, no. 11, article e471, 2009.
- J. W. Bailey and D. H. Smith, “The quantitative buffy coat for the diagnosis of trypanosomes,” Tropical Doctor, vol. 24, no. 2, pp. 54–56, 1994.
- T. W. Miézan, H. A. Meda, F. Doua, N. N. Djè, V. Lejon, and P. Büscher, “Single centrifugation of cerebrospinal fluid in a sealed Pasteur pipette for simple, rapid and sensitive detection of trypanosomes,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 94, no. 3, p. 293, 2000.
- P. Cattand, B. T. Miezan, and P. de Raadt, “Human African trypano somiasis: use of double centrifugation of cerebrospinal fluid to detect try panosomes,” Bulletin of the World Health Organisation, vol. 66, no. 1, pp. 83–86, 1988.
- N. N. Massamba and R. O. Williams, “Distinction of African trypanosome species using nucleic acid hybridization,” Parasitology, vol. 88, no. 1, pp. 55–65, 1984.
- O. K. Ole-MoiYoi, “Trypanosome species-specific DNA probe to detect infection in tsetse flies,” Parasitology Today, vol. 3, no. 12, pp. 371–374, 1987.
- R. K. Saiki, S. Scharf, and F. Faloona, “Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia,” Science, vol. 230, no. 4732, pp. 1350–1354, 1985.
- D. R. Moser, G. A. Cook, D. E. Ochs, C. P. Bailey, M. R. McKane, and J. E. Donelson, “Detection of Trypanosoma congolense and Trypanosoma brucei subspecies by DNA amplification using the polymerase chain reaction,” Parasitology, vol. 99, no. 1, pp. 57–66, 1989.
- D. K. Masiga, A. J. Smyth, P. Hayes, T. J. Bromidge, and W. C. Gibson, “Sensitive detection of trypanosomes in tsetse flies by DNA amplification,” International Journal for Parasitology, vol. 22, no. 7, pp. 909–918, 1992.
- S. Deborggraeve and P. Büscher, “Molecular diagnostics for sleeping sickness: what is the benefit for the patient?” The Lancet Infectious Diseases, vol. 10, no. 6, pp. 433–439, 2010.
- M. Radwanska, F. Claes, S. Magez et al., “Novel primer sequences for polymerase chain reaction-based detection of Trypanosoma brucei gambiense,” American Journal of Tropical Medicine and Hygiene, vol. 67, no. 3, pp. 289–295, 2002.
- M. Radwanska, M. Chamekh, L. Vanhamme et al., “The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense,” American Journal of Tropical Medicine and Hygiene, vol. 67, no. 6, pp. 684–690, 2002.
- W. Gibson, T. Backhouse, and A. Griffiths, “The human serum resistance associated gene is ubiquitous and conserved in Trypanosoma brucei rhodesiense throughout East Africa,” Infection, Genetics and Evolution, vol. 1, no. 3, pp. 207–214, 2002.
- K. Picozzi, E. M. Fèvre, M. Odiit et al., “Sleeping sickness in Uganda: a thin line between two fatal diseases,” British Medical Journal, vol. 331, no. 7527, pp. 1238–1241, 2005.
- N. Maina, K. J. Maina, P. Mäser, and R. Brun, “Genotypic and phenotypic characterization of Trypanosoma brucei gambiense isolates from Ibba, South Sudan, an area of high melarsoprol treatment failure rate,” Acta Tropica, vol. 104, no. 2-3, pp. 84–90, 2007.
- E. Matovu, I. Kuepfer, A. Boobo, S. Kibona, and C. Burri, “Comparative detection of trypanosomal DNA by loop-mediated isothermal amplification and PCR from flinders technology associates cards spotted with patient blood,” Journal of Clinical Microbiology, vol. 48, no. 6, pp. 2087–2090, 2010.
- S. L. Wastling, K. Picozzi, C. Wamboga et al., “Latent Trypanosoma brucei gambiense foci in Uganda: a silent epidemic in children and adults?” Parasitology, vol. 138, no. 12, pp. 1480–1487, 2011.
- S. Deborggraeve, F. Claes, T. Laurent et al., “Molecular dipstick test for diagnosis of sleeping sickness,” Journal of Clinical Microbiology, vol. 44, no. 8, pp. 2884–2889, 2006.
- J. Compton, “Nucleic acid sequence-based amplification,” Nature, vol. 350, no. 6313, pp. 91–92, 1991.
- C. M. Mugasa, G. J. Schoone, R. A. Ekangu, G. W. Lubega, P. A. Kager, and H. D. F. H. Schallig, “Detection of Trypanosoma brucei parasites in blood samples using real-time nucleic acid sequence-based amplification,” Diagnostic Microbiology and Infectious Disease, vol. 61, no. 4, pp. 440–445, 2008.
- C. M. Mugasa, T. Laurent, G. J. Schoone, P. A. Kager, G. W. Lubega, and H. D. F. H. Schallig, “Nucleic acid sequence-based amplification with oligochromatography for detection of Trypanosoma brucei in clinical samples,” Journal of Clinical Microbiology, vol. 47, no. 3, pp. 630–635, 2009.
- E. Matovu, C. M. Mugasa, R. Ali Ekangu et al., “Phase II evaluation of sensitivity and specificity of PCR and NASBA Followed by Oligochromatography for Diagnosis of Human African Trypanosomiasis in Clinical Samples from D.R. Congo and Uganda,” PLoS Neglected Tropical Diseases, vol. 4, no. 7, article e737, 2010.
- T. Notomi, H. Okayama, H. Masubuchi et al., “Loop-mediated isothermal amplification of DNA,” Nucleic Acids Research, vol. 28, no. 12, article E63, 2000.
- N. Kuboki, N. Inoue, T. Sakurai et al., “Loop-mediated isothermal amplification for detection of African trypanosomes,” Journal of Clinical Microbiology, vol. 41, no. 12, pp. 5517–5524, 2003.
- O. M. M. Thekisoe, N. Kuboki, A. Nambota et al., “Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis,” Acta Tropica, vol. 102, no. 3, pp. 182–189, 2007.
- Z. K. Njiru, A. S. J. Mikosza, E. Matovu et al., “African trypanosomiasis: sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA,” International Journal for Parasitology, vol. 38, no. 5, pp. 589–599, 2008.
- S. Bhattacharya, A. Bakre, and A. Bhattacharya, “Mobile genetic elements in protozoan parasites,” Journal of Genetics, vol. 81, no. 2, pp. 73–86, 2002.
- Z. K. Njiru, A. S.J. Mikosza, T. Armstrong, J. C. Enyaru, J. M. Ndung'u, and A. R. C. Thompson, “Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense,” PLoS Neglected Tropical Diseases, vol. 2, no. 2, article e147, 2008.
- Z. K. Njiru, R. Traub, J. O. Ouma, J. C. Enyaru, and E. Matovu, “Detection of group 1 Trypanosoma brucei gambiense by loop-mediated isothermal amplification,” Journal of Clinical Microbiology, vol. 49, no. 4, pp. 1530–1536, 2011.
- S. L. Wastling, K. Picozzi, A. S. L. Kakembo, and S. C. Welburn, “LAMP for human African trypanosomiasis: a comparative study of detection formats,” PLoS Neglected Tropical Diseases, vol. 4, no. 11, article e865, 2010.
- Z. K. Njiru, “Rapid and sensitive detection of human African trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick,” Diagnostic Microbiology and Infectious Disease, vol. 69, no. 2, pp. 205–209, 2011.
- R. E. Morty, L. Troeberg, J. C. Powers, S. Ono, J. D. Lonsdale-Eccles, and T. H. T. Coetzer, “Characterisation of the antitrypanosomal activity of peptidyl α-aminoalkyl phosphonate diphenyl esters,” Biochemical Pharmacology, vol. 60, no. 10, pp. 1497–1504, 2000.
- M. Mamani-Matsuda, J. Rambert, D. Malvy et al., “Quercetin induces apoptosis of Trypanosoma brucei gambiense and decreases the proinflammatory response of human macrophages,” Antimicrobial Agents and Chemotherapy, vol. 48, no. 3, pp. 924–929, 2004.
- V. Lejon, P. Buscher, N. H. Serna, E. Magnus, and N. Van Meirvenne, “Human African trypanosomiasis: a latex agglutination field test for quantifying IgM in cerebrospinal fluid,” Bulletin of the World Health Organization, vol. 76, no. 6, pp. 553–558, 1998.
- A. Hainard, N. Tiberti, X. Robin et al., “A combined CXCL10, CXCL8 and H-FABP panel for the staging of human African trypanosomiasis patients,” PLoS Neglected Tropical Diseases, vol. 3, no. 6, article e459, 2009.
- N. Tiberti, A. Hainard, V. Lejon et al., “Discovery and verification of osteopontin and beta-2-microglobulin as promising markers for staging human African trypanosomiasis,” Molecular and Cellular Proteomics, vol. 9, no. 12, pp. 2783–2795, 2010.
- A. Hainard, N. Tiberti, X. Robin et al., “Matrix metalloproteinase-9 and intercellular adhesion molecule 1 are powerful staging markers for human African trypanosomiasis,” Tropical Medicine and International Health, vol. 16, no. 1, pp. 119–126, 2011.
- V. Lejon, V. Jamonneau, P. Solano et al., “Detection of trypanosome-specific antibodies in saliva, towards non-invasive serological diagnosis of sleeping sickness,” Tropical Medicine and International Health, vol. 11, no. 5, pp. 620–627, 2006.
- S. Deborggraeve, V. Lejon, R. A. Ekangu et al., “Diagnostic accuracy of pcr in gambiense sleeping sickness diagnosis, staging and post-treatment follow-up: a 2-year longitudinal study,” PLoS Neglected Tropical Diseases, vol. 5, no. 2, article e972, 2011.
- D. Legros, S. Evans, F. Maiso, J. C. K. Enyaru, and D. Mbulamberi, “Risk factors for treatment failure after melarsoprol for Trypanosoma bruceigambiense trypanosomiasis in Uganda,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 93, no. 4, pp. 439–442, 1999.
- E. Matovu, J. C. K. Enyaru, D. Legros, C. Schmid, T. Seebeck, and R. Kaminsky, “Melarsoprol refractory T. b. gambiense from Omugo, north-western Uganda,” Tropical Medicine and International Health, vol. 6, no. 5, pp. 407–411, 2001.
- F. W. Jennings, D. D. Whitelaw, and P. H. Holmes, “The brain as a source of relapsing Trypanosoma brucei infection in mice after chemotherapy,” International Journal for Parasitology, vol. 9, no. 4, pp. 381–384, 1979.
- V. Lejon, J. Robays, F. X. N'Siesi et al., “Treatment failure related to intrathecal immunoglobulin M (IgM) synthesis, cerebrospinal fluid IgM, and interleukin-10 in patients with hemolymphatic-stage sleeping sickness,” Clinical and Vaccine Immunology, vol. 14, no. 6, pp. 732–737, 2007.
- V. Lejon, I. Roger, D. M. Ngoyi et al., “Novel markers for treatment outcome in late-stage Trypanosoma brucei gambiense trypanosomiasis,” Clinical Infectious Diseases, vol. 47, no. 1, pp. 15–22, 2008.
- D. M. Ngoyi, V. Lejon, P. Pyana et al., “How to shorten patient follow-up after treatment for Trypanosoma brucei gambiense sleeping sickness,” Journal of Infectious Diseases, vol. 201, no. 3, pp. 453–463, 2010.
- L. C. van Nieuwenhove, S. Rogé, F. Balharbi et al., “Identification of peptide mimotopes of Trypanosoma brucei gambiense variant surface Glycoproteins,” PLoS Neglected Tropical Diseases, vol. 5, no. 6, article e1189, 2011.
- G. D. Kanmogne, “Detection of Trypanosoma brucei gambiense, in serologically positive but aparasitaemic sleeping-sickness suspects in Cameroon, by PCR,” Annals of Tropical Medicine and Parasitology, vol. 90, no. 5, pp. 475–483, 1996.