Structure-Function of Falcipains: Malarial Cysteine Proteases

Figure 5

Expression and refolding of different constructs of falcipain-2. Different constructs were used to study refolding. The mature domain was expressed alone or with the refolding domain either in cis or trans. In the last construct, folding of mature domain was done in trans with profolding domain. After purification of the mature domain, the activity was assayed [29].