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Journal of Tropical Medicine
Volume 2012 (2012), Article ID 564721, 5 pages
http://dx.doi.org/10.1155/2012/564721
Research Article

Comparison of the Triage Micro Parasite Panel and Microscopy for the Detection of Entamoeba histolytica/Entamoeba dispar, Giardia lamblia, and Cryptosporidium parvum in Stool Samples Collected in Kenya

1Kenya Medical Research Institute/Walter Reed Project, United States Army Medical Research Unit—Kenya, P.O. Box 1357, Kericho 20200, Kenya
2Division of Bacterial and Rickettsial Diseases, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA

Received 28 March 2012; Revised 16 May 2012; Accepted 30 May 2012

Academic Editor: Blaise Genton

Copyright © 2012 Brett Swierczewski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. C. J. L. Murray and A. D. Lopez, “Mortality by cause for eight regions of the world: global burden of disease study,” The Lancet, vol. 349, no. 9061, pp. 1269–1276, 1997. View at Scopus
  2. R. L. Guerrant, J. M. Hughes, N. L. Lima, and J. Crane, “Diarrhea in developed and developing countries: magnitude, special settings, and etiologies,” Reviews of Infectious Diseases, vol. 12, supplement 1, pp. S41–S50, 1990. View at Scopus
  3. M. Kosek, C. Bern, and R. L. Guerrant, “The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000,” Bulletin of the World Health Organization, vol. 81, no. 3, pp. 197–204, 2003. View at Scopus
  4. C. L. F. Walker, M. J. Aryee, C. Boschi-Pinto, R. E. Black, et al., “Estimating diarrhea mortality among young children in low and middle income countries,” PLoS One, vol. 7, no. 1, Article ID e29151, 2012. View at Publisher · View at Google Scholar
  5. C. Kourenti, P. Karanis, and H. Smith, “Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt,” Journal of Water and Health, vol. 5, no. 1, pp. 1–38, 2007. View at Publisher · View at Google Scholar · View at Scopus
  6. P. C. Okhuysen, “Traveler's diarrhea due to intestinal protozoa,” Clinical Infectious Diseases, vol. 33, no. 1, pp. 110–114, 2001. View at Publisher · View at Google Scholar · View at Scopus
  7. T. G. Mank, J. O. M. Zaat, A. M. Deelder, J. T. M. Van Eijk, and A. M. Polderman, “Sensitivity of microscopy versus enzyme immunoassay in the laboratory diagnosis of giardiasis,” European Journal of Clinical Microbiology and Infectious Diseases, vol. 16, no. 8, pp. 615–619, 1997. View at Publisher · View at Google Scholar · View at Scopus
  8. M. M. Marshall, D. Naumovitz, Y. Ortega, and C. R. Sterling, “Waterborne protozoan pathogens,” Clinical Microbiology Reviews, vol. 10, no. 1, pp. 67–85, 1997. View at Scopus
  9. S. P. Johnston, M. M. Ballard, M. J. Beach, L. Causer, and P. P. Wilkins, “Evaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens,” Journal of Clinical Microbiology, vol. 41, no. 2, pp. 623–626, 2003. View at Publisher · View at Google Scholar · View at Scopus
  10. R. A. Nesbitt, F. W. Mosha, H. A. Katki, M. Ashraf, C. Assenga, and C. M. Lee, “Amebiasis and comparison of microscopy to ELISA technique in detection of Entamoeba histolytica and Entamoeba dispar,” Journal of the National Medical Association, vol. 96, no. 5, pp. 671–677, 2004. View at Scopus
  11. A. Kebede, J. Verweij, W. Dorigo-Zetsma et al., “Overdiagnosis of amoebiasis in the absence of Entamoeba histolytica among patients presenting with diarrhoea in Wonji and Akaki, Ethiopia,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 97, no. 3, pp. 305–307, 2003. View at Publisher · View at Google Scholar · View at Scopus
  12. A. Kebede, J. J. Verweij, B. Petros, and A. M. Polderman, “Short communication: misleading microscopy in amoebiasis,” Tropical Medicine and International Health, vol. 9, no. 5, pp. 651–652, 2004. View at Publisher · View at Google Scholar · View at Scopus
  13. M. Danciger and M. Lopez, “Numbers of Giardia in the feces of infected children,” American Journal of Tropical Medicine and Hygiene, vol. 24, no. 2, pp. 237–242, 1975. View at Scopus
  14. R. D. Newman, K. L. Jaeger, T. Wuhib, A. A. M. Lima, R. L. Guerrant, and C. L. Sears, “Evaluation of an antigen capture enzyme-linked immunosorbent assay for detection of Cryptosporidium oocysts,” Journal of Clinical Microbiology, vol. 31, no. 8, pp. 2080–2084, 1993. View at Scopus
  15. L. S. Garcia and R. Y. Shimizu, “Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens,” Journal of Clinical Microbiology, vol. 35, no. 6, pp. 1526–1529, 1997. View at Scopus
  16. R. Haque, I. K. M. Ali, S. Akther, and W. A. Petri, “Comparison of PCR, isoenzyme analysis, and antigen detection for diagnosis of Entamoeba histolytica infection,” Journal of Clinical Microbiology, vol. 36, no. 2, pp. 449–452, 1998. View at Scopus
  17. S. Buss, M. Kabir, W. A. Petri, and R. Haque, “Comparison of two immunoassays for detection of Entamoeba histolytica,” Journal of Clinical Microbiology, vol. 46, no. 8, pp. 2778–2779, 2008. View at Publisher · View at Google Scholar · View at Scopus
  18. L. S. Garcia, R. Y. Shimizu, and C. N. Bernard, “Detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum antigens in human fecal specimens using the triage parasite panel enzyme immunoassay,” Journal of Clinical Microbiology, vol. 38, no. 9, pp. 3337–3340, 2000. View at Scopus
  19. S. E. Sharp, C. A. Suarez, Y. Duran, and R. J. Poppiti, “Evaluation of the triage micro parasite panel for detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum in patient stool specimens,” Journal of Clinical Microbiology, vol. 39, no. 1, pp. 332–334, 2001. View at Publisher · View at Google Scholar · View at Scopus
  20. B. Leiva, M. Lebbad, J. Winiecka-Krusnell, I. Altamirano, A. Tellez, and E. Linder, “Overdiagnosis of Entamoeba histolytica and Entamoeba dispar in Nicaragua: a microscopic, triage parasite panel and PCR study,” Archives of Medical Research, vol. 37, no. 4, pp. 529–534, 2006. View at Publisher · View at Google Scholar · View at Scopus
  21. M. R. Gaafar, “Evaluation of enzyme immunoassay techniques for diagnosis of the most common intestinal protozoa in fecal samples,” International Journal of Infectious Diseases, vol. 15, no. 8, pp. e541–e544, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. L. S. Garcia, Diagnostic Medical Parasitology, American Society for Microbiology, Washington, DC, USA, 4th edition, 2001.
  23. R. G. Knapp and M. C. Miller, Clinical Epidemiology and Biostatistics, Harwal, Baltimore, Md, USA, 1992.
  24. J. A. Platts-Mills, J. Darwin, E. R. Houpt, et al., “Molecular diagnosis of diarrhea: current status and future potential,” Current Infectious Disease Reports, vol. 14, no. 1, pp. 41–46, 2012. View at Publisher · View at Google Scholar
  25. A. M. van Eijk, J. T. Brooks, P. M. Adcock et al., “Diarrhea in children less than two years of age with known HIV status in Kisumu, Kenya,” International Journal of Infectious Diseases, vol. 14, no. 3, pp. e220–e225, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. R. N. Chunge, N. Nagelkerke, P. N. Karumba et al., “Longitudinal study of young children in Kenya: intestinal parasitic infection with special reference to Giarida lamblia, its prevalence, incidence and duration, and its association with diarrhoea and with other parasites,” Acta Tropica, vol. 50, no. 1, pp. 39–49, 1991. View at Publisher · View at Google Scholar · View at Scopus
  27. W. Gatei, C. N. Wamae, C. Mbae et al., “Cryptosporidiosis: prevalence, genotype analysis, and symptoms associated with infections in children in Kenya,” American Journal of Tropical Medicine and Hygiene, vol. 75, no. 1, pp. 78–82, 2006. View at Scopus
  28. D. R. Pillai, J. S. Keystone, D. C. Sheppard, J. D. MacLean, D. W. MacPherson, and K. C. Kain, “Entamoeba histolytica and Entamoeba dispar: epidemiology and comparison of diagnostic methods in a setting of nonendemicity,” Clinical Infectious Diseases, vol. 29, no. 5, pp. 1315–1318, 1999. View at Publisher · View at Google Scholar · View at Scopus
  29. R. M. Chalmers, “Cryptosporidium: from laboratory diagnosis to surveillance and outbreaks,” Parasite, vol. 15, no. 3, pp. 372–378, 2008. View at Scopus
  30. H. L. DuPont, C. L. Chappell, C. R. Sterling, P. C. Okhuysen, J. B. Rose, and W. Jakubowski, “The infectivity of Cryptosporidium parvum in healthy volunteers,” New England Journal of Medicine, vol. 332, no. 13, pp. 855–859, 1995. View at Publisher · View at Google Scholar · View at Scopus
  31. L. S. Diamond and C. G. Clark, “A redescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925,” The Journal of Eukaryotic Microbiology, vol. 40, no. 3, pp. 340–344, 1993. View at Scopus
  32. P. Gonin and L. Trudel, “Detection and differentiation of Entamoeba histolytica and Entamoeba dispar isolates in clinical samples by PCR and enzyme-linked immunosorbent assay,” Journal of Clinical Microbiology, vol. 41, no. 1, pp. 237–241, 2003. View at Publisher · View at Google Scholar · View at Scopus
  33. P. G. Sargeaunt and J. E. Williams, “Electrophoretic isoenzyme patterns of the pathogenic and non-pathogenic intestinal amoebae of man,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 73, no. 2, pp. 225–227, 1979. View at Scopus
  34. M. Tanyuksel and W. A. Petri Jr, “Laboratory diagnosis of amebiasis,” Clinical Microbiology Reviews, vol. 16, no. 4, pp. 713–729, 2003. View at Publisher · View at Google Scholar · View at Scopus
  35. N. C. Christy, J. D. Hencke, A. Escueta-De Cadiz, et al., “Multisite performance evaluation of an enzyme-linked immunosorbent assay for detection of giardia, Cryptosporidium, and Entamoeba histolytica antigens in human stool,” Journal of Clinical Microbiology, vol. 50, no. 5, pp. 1762–1763, 2012.
  36. R. Haque, S. Roy, A. Siddique et al., “Multiplex real-time PCR assay for detection of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp,” American Journal of Tropical Medicine and Hygiene, vol. 76, no. 4, pp. 713–717, 2007. View at Scopus