Moving “Forward” in Plasmodium Genetics through a Transposon-Based Approach

Figure 2

Basic plasmid designs for piggyBac mutagenesis in Plasmodium. The donor plasmid consists of a Plasmodium drug selection cassette flanked by the terminal repeats (TR) of piggyBac. The helper plasmid provides the piggyBac transposase, which mediates the transposition event into the Plasmodium genome upon cotransfection with the donor plasmid.