Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease

Table 1

Sensitivity, specificity, positive predictive values, and negative predictive values of the ESEA-ELISA at various arbitrary OD cut-off values.

OD cut-off; Values and parameters	ESEA-based assay
0.400
Specificity (%) (95% CI)	74 (62–86)
Sensitivity (%) (95% CI)	100
NPV (%) (95% CI)	100
PPV (%) (95% CI)	79 (69–89)
0.500
Specificity (%) (95% CI)	80 (69–91)
Sensitivity (%) (95% CI)	98 (94–100)
NPV (%) (95% CI)	98 (93–100)
PPV (%) (95% CI)	83 (73–93)
0.600
Specificity (%) (95% CI)	88 (79–97)
Sensitivity (%) (95% CI)	98 (96–100)
NPV (%) (95% CI)	98 (94–100)
PPV (%) (95% CI)	89 (81–97)

NPV: negative predictive value.
PPV: positive predictive value.
CI: confidence interval.