Comparative Evaluation of Permissiveness to Dengue Virus Serotype 2 Infection in Primary Rodent Macrophages
Figure 2
qRT-PCR to obtain relative quantification of viral copies obtained from monolayers from different rodents’ peritoneal macrophages cultures. The fold change of viral RNA was calculated from DENV-infected macrophage monolayers, normalising the data obtained against noninfected control and against β-actin at 0,1 MOI (a) and at 1 MOI (b) and evaluated 24, 48, and 72 hours p.i. , .