Molecular Typing of Dengue Virus Circulating in Kolkata, India in 2010

Figure 3

Results of dengue-specific RT-PCR followed by second-round nested PCR of RNA samples, showing band in 1.5% agarose gel electrophoresis stained with ethidium bromide. Lane L: 100 bp DNA ladder, Lane S1–S8 and S10: negative sample, Lane S9, S14 and S20: sample positive for DEN-2 (119 bp), Lane S11–S13 and S16–S19: sample with dual infection of DEN-2 (119 bp) and DEN-3 (290 bp), Lane S15: sample with concurrent infection of DEN-1 (482 bp), DEN-2 (119 bp), and DEN-3 (290 bp), and Lane C: negative control.