Challenges in Laboratory Diagnosis of Malaria in a Low-Resource Country at Tertiary Care in Eastern Nepal: A Comparative Study of Conventional vs. Molecular Methodologies

Table 3

Summary table representing the comparison of real-time PCR cycle threshold (Ct) value with the positive results of microscopy and the RDT.

Ct value (ranges)	Microscopy detection rate	RDT detection rate
<20 (n = 2)	2 (100%)	2 (100%)
20–25 (n = 2)	1 (50%)	2 (100%)
26–30 (n = 1)	Not detected	Not detected
31–35 (n = 2)	Not detected	Not detected
36–40 (n = 6)	Not detected	Not detected
Mean Ct value ± standard deviation; 30.30 ± 7.6	18.31 ± 3.86 ( value = 0.029)	19.66 ± 2.03 ( value = 0.016)