Isolation, Purification, and Characterization of Homogenous Novel Bioactive Protein from Datura stramonium Stem Exhibited Larvicidal Activity against Anopheles stephensi

Table 2

Percentage mortality of 3^rd instar larvae of An. stephensi at different concentrations of dialyzed crude and purified protein extract.

Protein extract	Concentration (mg/ml)	Period of exposure (hrs)
		24 h ± S.D.	48 h ± S.D.	72 h ± S.D.
Crude protein	0.172	20 ± 5	20 ± 5	26.6 ± 5.8
	0.34	36.6 ± 4.0	38.3 ± 3.5	40 ± 0.0
	0.68	48.3 ± 2.9	53.3 ± 2.7	53.3 ± 2.8
	1.37	60 ± 0.0	61.6 ± 3.5	65 ± 0.0
	2.75	73.3 ± 2.9	73.3 ± 2.8	75 ± 0.0
	5.5	85 ± 3.5	88.3 ± 3.2	95 ± 0.0
Purified protein	0.01	10 ± 0.0	10 ± 0.0
	0.02	26.6 ± 3.19	30 ± 0.0
	0.03	46.6 ± 7.02	50 ± 0.0
	0.04	60 ± 0.0	66.6 ± 8.16
	0.05	80 ± 0.0	81.6 ± 4.08
	0.06	90 ± 0.0	90 ± 0.0