Phenolics, Antioxidant and Antibacterial Activities of Immature and Mature Blumea balsamifera Leaf Extracts Eluted with Different Solvents
Table 4
Antioxidant capacity (DPPH, ABTS, FRAP, and nitric oxide scavenging) of immature and mature B. balsamifera leaf extracts obtained using different eluting solvents.
Treatment
DPPH
ABTS
FRAP
NO scavenging activity (μmol NO2/g DW)
Maturity
Solvent
(mg trolox/g DW)
Immature
57.78 ± 53.27a
46.30 ± 40.78a
56.27 ± 52.25a
788.08 ± 439.66a
Mature
45.75 ± 52.75b
32.63 ± 33.71b
40.91 ± 43.86b
651.21 ± 361.37b
F-test (maturity)
Water
40.76 ± 13.05b
28.85 ± 10.28b
39.90 ± 11.85b
759.75 ± 58.96c
50% EtOH
135.58 ± 8.88a
98.27 ± 13.18a
124.48 ± 15.61a
1158.48 ± 99.02a
95% EtOH
28.93 ± 9.60c
27.40 ± 7.75b
27.41 ± 7.52c
841.43 ± 193.90b
Hexane
1.80 ± 15.22d
3.35 ± 0.80c
2.57 ± 0.72d
118.92 ± 16.44d
F-test (solvent)
F-test (maturity solvent)
Immature
Water
52.48 ± 2.48b
37.88 ± 2.14c
50.41 ± 4.38c
809.34 ± 29.96c
50% EtOH
139.93 ± 8.47a
109.54 ± 5.98a
137.49 ± 8.91a
1222.52 ± 58.32a
95% EtOH
36.55 ± 6.61c
34.08 ± 2.88c
33.98 ± 2.38d
1014.29 ± 26.02b
Hexane
2.15 ± 0.07e
3.70 ± 0.77e
3.22 ± 0.17f
106.16 ± 10.15e
Mature
Water
29.04 ± 2.70 cd
19.81 ± 3.81d
29.39 ± 0.99d
710.15 ± 20.32d
50% EtOH
131.22 ± 8.27a
87.00 ± 4.19b
111.47 ± 4.73b
1094.45 ± 93.86b
95% EtOH
21.31 ± 3.49d
20.71 ± 2.76d
20.84 ± 2.48e
668.57 ± 60.60d
Hexane
1.44 ± 0.56e
3.00 ± 0.79e
1.92 ± 0.04f
131.67 ± 9.21e
F-test
C.V. (%)
9.89
8.47
8.41
6.6
Data are expressed as mean ± SD (n = 3). Values in the same column followed by different letters indicate significant differences at according to Duncan’s multiple range test; = significant at level.