Research Article

Phenolics, Antioxidant and Antibacterial Activities of Immature and Mature Blumea balsamifera Leaf Extracts Eluted with Different Solvents

Table 4

Antioxidant capacity (DPPH, ABTS, FRAP, and nitric oxide scavenging) of immature and mature B. balsamifera leaf extracts obtained using different eluting solvents.

TreatmentDPPHABTSFRAPNO scavenging activity (μmol NO2/g DW)
MaturitySolvent(mg trolox/g DW)

Immature57.78 ± 53.27a46.30 ± 40.78a56.27 ± 52.25a788.08 ± 439.66a
Mature45.75 ± 52.75b32.63 ± 33.71b40.91 ± 43.86b651.21 ± 361.37b
F-test (maturity)
Water40.76 ± 13.05b28.85 ± 10.28b39.90 ± 11.85b759.75 ± 58.96c
50% EtOH135.58 ± 8.88a98.27 ± 13.18a124.48 ± 15.61a1158.48 ± 99.02a
95% EtOH28.93 ± 9.60c27.40 ± 7.75b27.41 ± 7.52c841.43 ± 193.90b
Hexane1.80 ± 15.22d3.35 ± 0.80c2.57 ± 0.72d118.92 ± 16.44d
F-test (solvent)
F-test (maturity  solvent)
ImmatureWater52.48 ± 2.48b37.88 ± 2.14c50.41 ± 4.38c809.34 ± 29.96c
50% EtOH139.93 ± 8.47a109.54 ± 5.98a137.49 ± 8.91a1222.52 ± 58.32a
95% EtOH36.55 ± 6.61c34.08 ± 2.88c33.98 ± 2.38d1014.29 ± 26.02b
Hexane2.15 ± 0.07e3.70 ± 0.77e3.22 ± 0.17f106.16 ± 10.15e
MatureWater29.04 ± 2.70 cd19.81 ± 3.81d29.39 ± 0.99d710.15 ± 20.32d
50% EtOH131.22 ± 8.27a87.00 ± 4.19b111.47 ± 4.73b1094.45 ± 93.86b
95% EtOH21.31 ± 3.49d20.71 ± 2.76d20.84 ± 2.48e668.57 ± 60.60d
Hexane1.44 ± 0.56e3.00 ± 0.79e1.92 ± 0.04f131.67 ± 9.21e
F-test
C.V. (%)9.898.478.416.6

Data are expressed as mean ± SD (n = 3). Values in the same column followed by different letters indicate significant differences at according to Duncan’s multiple range test;  = significant at level.