Biochemical and Pharmacological Characterization of TLBbar, a New Serine Protease with Coagulant Activity from Bothrops barnetti Snake Venom

Figure 7

Fibrinogenolytic activity of TLBbar. (a) Time of incubation. (1) Control bovine fibrinogen 1.5 mg/mL; (2) 15 min; (3) 30 min; (4) 60 min; (5) 2 hours; (6) 4 hours; (7) 6 hours; (8) 24 hours. (b) Dose dependent. (1) Control bovine fibrinogen 1.5 mg/mL; (2) 1.25 μg TLBbar; (3) 2.5 μg TLBbar; (4) 5 μg TLBbar; (5) 10 μg TLBbar; (6) 20 μg TLBbar. (c) Temperature. (1) Control bovine fibrinogen 1.5 mg/mL; (2) 20°C; (3) 30°C; (4) 40°C; (5) 50°C; (6) 60°C; (7) 70°C; (8) 80°C. (d) Ions. (1) Control of bovine fibrinogen 1.5 mg/mL; (2) TLBbar; (3) TLBbar with Ca²⁺; (4) TLBbar with Mg²⁺; (5) TLBbar with Ba²⁺; (6) TLBbar with Zn²⁺; (7) TLBbar with Cu²⁺; (8) TLBbar with Cd²⁺. (e) Inhibition. (1) Control bovine fibrinogen 1.5 mg/mL; (2) TLBbar; (3) Leupeptin; (4) PMSF; (5) EDTA; (6) EGTA; (7) Heparin.

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